UBE2J2/UBC6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-06347

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human UBE2J2/UBC6 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for UBE2J2/UBC6 Antibody - BSA Free

Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347]

Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347]

Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347] - Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa
Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347]

Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347]

Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347] - UBE2J1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-UBE2J1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-UBE2J1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDa

Applications for UBE2J2/UBC6 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2J2

UBE2J2 - ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)

Long Name

Ubiquitin-conjugating Enzyme E2, J2

Alternate Names

NCUBE2, PRO2121, Ubc6, Ubc6p

Gene Symbol

UBE2J2

Additional UBE2J2 Products

Product Documents for UBE2J2/UBC6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UBE2J2/UBC6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail