UBE2S Antibody (OTI1B5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74749

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B5

Format

Azide and BSA Free
View all UBE2S Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2S(NP_055316) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2S Antibody (OTI1B5) - Azide and BSA Free

Western Blot: UBE2S Antibody (OTI1B5)Azide and BSA Free [NBP2-74749]

Western Blot: UBE2S Antibody (OTI1B5)Azide and BSA Free [NBP2-74749]

Western Blot: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S.
Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by UBE2S antibody.
Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of Hela cells, using UBE2S antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749]

Flow Cytometry: UBE2S Antibody (OTI1B5) - Azide and BSA Free [NBP2-74749] - Analysis of Jurkat cells, using UBE2S antibody,(Red), compared to a nonspecific negative control antibody (Blue).

Applications for UBE2S Antibody (OTI1B5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2S

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Long Name

Ubiquitin-conjugating Enzyme E2S

Alternate Names

E2-24K, E2-EPF, E2-EPF52, EPF5

Gene Symbol

UBE2S

Additional UBE2S Products

Product Documents for UBE2S Antibody (OTI1B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2S Antibody (OTI1B5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail