![UBE3B Antibody (OTI3F10) Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-](https://resources.rndsystems.com/images/products/nbp3-44687_mouse-ube3b-mab-oti3f10-azide-and-bsa-free-immunohistochemistry-75202622443322.jpg "Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3F10
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 768-1068 of human UBE3B (NP_569733) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for UBE3B Antibody (OTI3F10)
Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-UBE3B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![UBE3B Antibody (OTI3F10) Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-](https://resources.rndsystems.com/images/products/nbp3-44687_mouse-ube3b-mab-oti3f10-azide-and-bsa-free-immunohistochemistry-752026237595.jpg "Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-")
Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-
Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-UBE3B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![UBE3B Antibody (OTI3F10) Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-](https://resources.rndsystems.com/images/products/nbp3-44687_mouse-ube3b-mab-oti3f10-azide-and-bsa-free-immunohistochemistry-7520262245247.jpg "Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-")
Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE3B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.![UBE3B Antibody (OTI3F10) Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-](https://resources.rndsystems.com/images/products/nbp3-44687_mouse-ube3b-mab-oti3f10-azide-and-bsa-free-immunohistochemistry-752026234495.jpg "Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-")
Immunohistochemistry;UBE3B Antibody (OTI3F10)[NBP3-44687]-
Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-UBE3B mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.Applications for UBE3B Antibody (OTI3F10)
Application
Recommended Usage
Immunohistochemistry
1:250
Formulation, Preparation, and Storage
Purification
>95%
Reconstitution
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized powder ( 1X PBS, pH 7.3,)
Preservative
8% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Calculators
Background: UBE3B
Alternate Names
DKFZp586K2123, DKFZp686A1051, EC 6.3.2, EC 6.3.2.-, FLJ45294, MGC131858, MGC78388, ubiquitin protein ligase E3B, ubiquitin-protein ligase E3B
Gene Symbol
UBE3B
Additional UBE3B Products
Product Documents for UBE3B Antibody (OTI3F10)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UBE3B Antibody (OTI3F10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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