UBE4B Antibody

Novus Biologicals | Catalog # NB100-58802

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all UBE4B Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human ubiquitination factor E4B using the numbering given in entry AAD02233.1 (GeneID 10277).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE4B Antibody

Western Blot: UBE4B Antibody [NB100-58802]

Western Blot: UBE4B Antibody [NB100-58802]

Western Blot: UBE4B Antibody [NB100-58802] - Detection of mouse UBE4B by western blot. Samples: Nuclear extract (50 ug) from NIH 3T3, TCMK-1, and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: UBE4B Antibody [NB100-58802]

Western Blot: UBE4B Antibody [NB100-58802]

Western Blot: UBE4B Antibody [NB100-58802] - Detection of Human UBE4B by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: UBE4B Antibody [NB100-58802]

Immunoprecipitation: UBE4B Antibody [NB100-58802]

Immunoprecipitation: UBE4B Antibody [NB100-58802] - Detection of human UBE4B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for IP at 6 ug per reaction. UBE4B was also immunoprecipitated by rabbit anti-UBE4B antibody NB100-58803. For blotting immunoprecipitated UBE4B, NB100-58802 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for UBE4B Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBE4B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes.

Long Name

Ubiquitination Factor E4B

Alternate Names

UBOX3, UFD2, UFD2a

Entrez Gene IDs

10277 (Human); 63958 (Mouse)

Gene Symbol

UBE4B

UniProt

AAD02233.1 (Human)

Additional UBE4B Products

Product Documents for UBE4B Antibody

Certificate of Analysis

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Product Specific Notices for UBE4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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