Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05789

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 05

Format

Azide and BSA Free
View all Ubiquitin-activating Enzyme/UBE1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ubiquitin-activating Enzyme/UBE1 (Accession#: NP_003325.2; Ser2-Arg1058).

Reactivity Notes

No cross-reactivity in ELISA with:

Insect cell lysate

Specificity

No cross-reactivity in ELISA with: Insect cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] -

Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - UBA1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5 uL NBP3-05789 and 60 ug of Immunomagnetic beads Protein G. Primary antibody: NBP3-05789, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 111 kDa Observed band size: 111 kDa

Applications for Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.2-1 ul/mg of lysate

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS (pH 7.0)

Format

Azide and BSA Free

Preservative

10% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquitin-activating Enzyme/UBE1

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Alternate Names

A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme

Gene Symbol

UBA1

Additional Ubiquitin-activating Enzyme/UBE1 Products

Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E1 Ubiquitin/Ubl Activating Enzymes

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail