UBR5/EDD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-71802

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBR5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 2749 and 2799 of human E3 Ubiquitin Protein Ligase, HECT domain using the numbering given in entry NP_056986.2 (GeneID 51366).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBR5/EDD Antibody - BSA Free

UBR5/EDD Antibody [NBP1-71802] - Detection of human and mouse EDD1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (H; 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (5, 15, and 50 ug for WB) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EDD1 antibody NBP1-71802 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. EDD1 was less efficiently immunoprecipitated by another rabbit anti-EDD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for UBR5/EDD Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBR5

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Long Name

Ubiquitin Protein Ligase E3 Component N-recognin 5

Alternate Names

DD5, EDD, EDD1, HYD

Gene Symbol

UBR5

UniProt

NP_056986.2 (Human)

Additional UBR5 Products

Product Documents for UBR5/EDD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBR5/EDD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Customer Reviews for UBR5/EDD Antibody - BSA Free

There are currently no reviews for this product. Be the first to review UBR5/EDD Antibody - BSA Free and earn rewards!

Have you used UBR5/EDD Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies