UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

Novus Biologicals | Catalog # NBP2-46621

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3210

Format

BSA Free
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P09936, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Staining of U-251 cells using the Anti-UCHL1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human fallopian tube shows absence of immunoreactivity (negative control).
Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251 AAb
Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of rat cerebral cortex shows moderate immunoreactivity in neurons.
Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of mouse trigeminal ganglion shows strong immunoreactivity in primary sensory neurons.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human cerebellum shows moderate positivity in Purkinje cells.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human prostate shows staining in peripheral nerve fibers.
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human duodenum shows staining in peripheral nerve fibers.

Applications for UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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