UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43791
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3210
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P09936, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free
![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202512135822.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251 AAb
![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-28220251218277.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202512154124.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Western Blot: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue
![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202512154151.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of human cerebellum shows moderate positivity in Purkinje cells.![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202512321053.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of human duodenum shows staining in peripheral nerve fibers.![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-28220251238427.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of human fallopian tube shows absence of immunoreactivity (negative control).![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-28220251344272.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of human prostate shows staining in peripheral nerve fibers.![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202513401794.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of rat cerebral cortex shows moderate immunoreactivity in neurons.![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-28220251337856.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of mouse trigeminal ganglion shows strong immunoreactivity in primary sensory neurons.![UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP3-43791]](https://resources.rndsystems.com/images/products/nbp3-43791_mouse-uch-l1-pgp9-5-mab-cl3210-azide-and-bsa-free-282202513355857.jpg "Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP3-43791]")
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody [NBP3-43791]
Staining of U-251 cells using the Anti-UCH-L1/PGP9.5 monoclonal antibody).Applications for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: UCH-L1/PGP9.5
UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.
Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.
Long Name
Ubiquitin C-terminal Hydrolase L1
Alternate Names
PARK5, PGP9.5, UCHL1
Gene Symbol
UCHL1
Additional UCH-L1/PGP9.5 Products
Product Documents for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UCH-L1/PGP9.5 Antibody (CL3210) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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