UCP2 Antibody (C1) - BSA Free
Novus Biologicals | Catalog # NBP3-41696
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # C1
Format
BSA Free
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Product Specifications
Immunogen
Immunized with recombinant Human UCP2 (Uniprot#: P55851; Arg154-Val289 (Uniprot#: P55851)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Novus Biologicals Mouse UCP2 Antibody (C1) - BSA Free (NBP3-41696) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for UCP2 Antibody (C1) - BSA Free
Immunohistochemistry-Paraffin: UCP2 Antibody (C1) [NBP3-41696] -
Immunohistochemistry-Paraffin: UCP2 Antibody (C1) [NBP3-41696] - DAB staining on IHC-P; Samples: Human Stomach Tissue
Western Blot: UCP2 Antibody (C1) [NBP3-41696] -
Western Blot: UCP2 Antibody (C1) [NBP3-41696] - Sample: Recombinant UCP2, Human.
Immunohistochemistry-Paraffin: UCP2 Antibody (C1) [NBP3-41696] -
Immunohistochemistry-Paraffin: UCP2 Antibody (C1) [NBP3-41696] - DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30ug/ml NBP3-41696; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyApplications for UCP2 Antibody (C1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
5-30ug/mL
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01M PBS, pH7.4, 50% glycerol
Format
BSA Free
Preservative
0.05% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UCP2
Long Name
Uncoupling Protein 2
Alternate Names
SLC25A8, UCPH
Gene Symbol
UCP2
Additional UCP2 Products
Product Documents for UCP2 Antibody (C1) - BSA Free
Product Specific Notices for UCP2 Antibody (C1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for UCP2 Antibody (C1) - BSA Free
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Q: Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
A: Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.
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