UCP3 Antibody

Novus Biologicals | Catalog # NBP2-24608

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Mouse

Predicted:

Canine (90%), Porcine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A portion of amino acids 125-175 of human UCP3 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 84% homologous in bovine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UCP3 Antibody

Western Blot: UCP3 Antibody [NBP2-24608]

Western Blot: UCP3 Antibody [NBP2-24608]

Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart
UCP3 Antibody

Western Blot: UCP3 Antibody [NBP2-24608] -

Effect of CLA on the pWAT of mice induced with a HFD for 16 w. (A) Representative H and E staining of pWAT sections. Original magnification, × 400. Scale bar, 50 μm. (B) Adipocyte diameter and (C) Adipocyte area are shown (n = 6). (D) Representative transmission electronic microscopy images of pWAT. M, mitochondria; original magnification, × 0.7 k and × 3.0 k. Scale bar, 10.0 μm. (E) Detection of Caveolin-1, MT and UCP3 by Western blotting (n = 3 in each group). (F) Representative fluorescence characterization of LipidTOX for detection of the adipocytes. (G) Representative fluorescence characterization of PECAM-1 for the detection of angiogenesis. Original magnification, × 200. Scale bar, 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34135763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
UCP3 Antibody

Western Blot: UCP3 Antibody [NBP2-24608] -

Measurement of protein expression for common molecular pathways associated with muscle wasting.(A–H) Western blotting results evaluating muscle proteins involved in ubiquitination (A and B, left), autophagy (C and D), anabolism (E and F), and mitochondria biogenesis and metabolism (G and H) from protein lysates made from quadriceps harvested at euthanasia. Results are from a single experiment with no tumor (n = 5), KPC (n = 6), and KPC IL6KO (n = 5) tumor mice, and statistical differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) were determined using ANOVA and Tukey’s multiple comparisons test. Analysis of qPCR results for mRNA expression of E3 ubiquitin ligases Atrogin-1 and Murf1 in quadriceps was also performed (B, right). Data are from a single experiment with n = 5 per group, and statistical differences (***, P < 0.0001) were determined with ANOVA and Tukey’s multiple comparisons test. Error bars are mean and SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33851955), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for UCP3 Antibody

Application
Recommended Usage

Western Blot

0.5-2.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCP3

The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissue of mammals. UCP 1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.

Long Name

Uncoupling Protein 3 (Mitochondrial, Proton Carrier)

Alternate Names

SLC25A9

Gene Symbol

UCP3

UniProt

Additional UCP3 Products

Product Documents for UCP3 Antibody

Certificate of Analysis

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Product Specific Notices for UCP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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