UCP3 Antibody
Novus Biologicals | Catalog # NBP2-24608
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Cited:
Mouse
Predicted:
Canine (90%), Porcine (94%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 125-175 of human UCP3 was used as the immunogen.
Reactivity Notes
The amino acid sequence used as immunogen is 84% homologous in bovine.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UCP3 Antibody
Western Blot: UCP3 Antibody [NBP2-24608]
Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heartWestern Blot: UCP3 Antibody [NBP2-24608] -
Effect of CLA on the pWAT of mice induced with a HFD for 16 w. (A) Representative H and E staining of pWAT sections. Original magnification, × 400. Scale bar, 50 μm. (B) Adipocyte diameter and (C) Adipocyte area are shown (n = 6). (D) Representative transmission electronic microscopy images of pWAT. M, mitochondria; original magnification, × 0.7 k and × 3.0 k. Scale bar, 10.0 μm. (E) Detection of Caveolin-1, MT and UCP3 by Western blotting (n = 3 in each group). (F) Representative fluorescence characterization of LipidTOX for detection of the adipocytes. (G) Representative fluorescence characterization of PECAM-1 for the detection of angiogenesis. Original magnification, × 200. Scale bar, 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34135763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: UCP3 Antibody [NBP2-24608] -
Measurement of protein expression for common molecular pathways associated with muscle wasting.(A–H) Western blotting results evaluating muscle proteins involved in ubiquitination (A and B, left), autophagy (C and D), anabolism (E and F), and mitochondria biogenesis and metabolism (G and H) from protein lysates made from quadriceps harvested at euthanasia. Results are from a single experiment with no tumor (n = 5), KPC (n = 6), and KPC IL6KO (n = 5) tumor mice, and statistical differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) were determined using ANOVA and Tukey’s multiple comparisons test. Analysis of qPCR results for mRNA expression of E3 ubiquitin ligases Atrogin-1 and Murf1 in quadriceps was also performed (B, right). Data are from a single experiment with n = 5 per group, and statistical differences (***, P < 0.0001) were determined with ANOVA and Tukey’s multiple comparisons test. Error bars are mean and SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33851955), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for UCP3 Antibody
Application
Recommended Usage
Western Blot
0.5-2.0 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UCP3
Long Name
Uncoupling Protein 3 (Mitochondrial, Proton Carrier)
Alternate Names
SLC25A9
Gene Symbol
UCP3
UniProt
Additional UCP3 Products
Product Documents for UCP3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UCP3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for UCP3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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