UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82087

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (94%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UFM1 Activating Enzyme/UBA5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunocytochemistry/ Immunofluorescence: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunocytochemistry/Immunofluorescence: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087]

Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Applications for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UFM1 Activating Enzyme/UBA5

UBA5 encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq]

Alternate Names

THIFP1, UBA5, UBE1DC1

Gene Symbol

UBA5

Additional UFM1 Activating Enzyme/UBA5 Products

Product Documents for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UFM1 Activating Enzyme/UBA5 Antibody - BSA Free

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Protocols

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