UNC119 Antibody (2F9-2A9) - Azide and BSA Free

Novus Biologicals | Catalog # H00009094-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F9-2A9

Format

Azide and BSA Free
View all UNC119 Primary Antibodies »

Product Specifications

Immunogen

UNC119 (AAH27176.1, 1 a.a. ~ 240 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP

Specificity

UNC119 - unc-119 homolog (C. elegans)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for UNC119 Antibody (2F9-2A9) - Azide and BSA Free

Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01] - Primary antibody: 1:1000, 4 degree, overnight. Secondary antibody: 1:3000 HRP-antimouse IgG 37 degree, 3 hr. This image was submitted via customer Review.
Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Western Blot: UNC119 Antibody (2F9-2A9) [H00009094-M01] - Analysis of UNC119 expression in transfected 293T cell line by UNC119 monoclonal antibody (M01), clone 2F9-2A9.Lane 1: UNC119 transfected lysate(27 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Immunoprecipitation: UNC119 Antibody (2F9-2A9) [H00009094-M01]

Immunoprecipitation: UNC119 Antibody (2F9-2A9) [H00009094-M01] - Analysis of UNC119 transfected lysate using anti-UNC119 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UNC119 purified MaxPab rabbit polyclonal antibody.

Applications for UNC119 Antibody (2F9-2A9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 3 using H00009094-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UNC119

This gene is specifically expressed in the photoreceptors in the retina. The encoded product shares strong homology with the C. elegans unc119 protein and it can functionally complement the C. elegans unc119 mutation. It has been localized to the photoreceptor synapses in the outer plexiform layer of the retina, and suggested to play a role in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. Two transcript variants encoding different isoforms have been described for this gene.

Long Name

Protein unc-119 homolog A

Alternate Names

hRG4, Retinal protein 4, RG4

Entrez Gene IDs

9094 (Human)

Gene Symbol

UNC119

OMIM

604011 (Human)

UniProt

AAH27176 (Human)

Additional UNC119 Products

Product Documents for UNC119 Antibody (2F9-2A9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UNC119 Antibody (2F9-2A9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UNC119 Antibody (2F9-2A9) - Azide and BSA Free

Customer Reviews for UNC119 Antibody (2F9-2A9) - Azide and BSA Free (1)

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Customer Images

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  • UNC119 Antibody (2F9-2A9)
    Name: Taira Mayanagi
    Application: Western Blot
    Sample Tested: mouse eye, mouse hippocampus and Cultured rat embryonic hippocampal neurons
    Species: Mouse and Rat
    Verified Customer | Posted 09/04/2017
    Western blotting Blocking: 5% Skim-milk TBS-T 1st Ab reaction: 1/1000 in Canget signal immunoenhancer solution (TOYOBO), 4 degree, overnight 2nd Ab reaction: 1/3000 HRP-antimouse IgG (GE healthcare) in Canget signal solution, 37 degree, 3 hr
    UNC119 Antibody (2F9-2A9) - Azide and BSA Free H00009094-M01

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