UNC5H3/UNC5C Antibody
Novus Biologicals | Catalog # NBP1-37002
![Immunocytochemistry/ Immunofluorescence: UNC5H3/UNC5C Antibody [NBP1-37002]](https://resources.rndsystems.com/images/products/UNC5H3-UNC5C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-37002-img0004.jpg "Immunocytochemistry/ Immunofluorescence: UNC5H3/UNC5C Antibody [NBP1-37002]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence NCTVSEEPTGID corresponding to internal region according to NP_003719.2.
Epitope
NCTVSEEPTGID
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for UNC5H3/UNC5C Antibody
Immunocytochemistry/ Immunofluorescence: UNC5H3/UNC5C Antibody [NBP1-37002]
Immunocytochemistry/Immunofluorescence: UNC5H3/UNC5C Antibody [NBP1-37002] - Staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.![Immunohistochemistry-Paraffin: UNC5H3/UNC5C Antibody [NBP1-37002]](https://resources.rndsystems.com/images/products/UNC5H3-UNC5C-Antibody-Immunohistochemistry-Paraffin-NBP1-37002-img0005.jpg "Immunohistochemistry-Paraffin: UNC5H3/UNC5C Antibody [NBP1-37002]")
Immunohistochemistry-Paraffin: UNC5H3/UNC5C Antibody [NBP1-37002]
Immunohistochemistry-Paraffin: UNC5H3/UNC5C Antibody [NBP1-37002] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/ Immunofluorescence: UNC5H3/UNC5C Antibody [NBP1-37002] -
Expression of UNC5c protein in the medial prefrontal cortex of an adult male mouse.Low (A) and high (B) magnification images demonstrate that there is little UNC5c expression in dopamine axons in the medial prefrontal cortex. Here, we identify dopamine axons by immunofluorescent staining for tyrosine hydroxylase (TH). See our Methods subsection ‘Immunohistochemistry’ for details. Abbreviations: fmi: forceps minor of the corpus callosum, mPFC: medial prefrontal cortex. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/88261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for UNC5H3/UNC5C Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
3 - 5 ug/ml
Immunohistochemistry-Paraffin
3 - 5 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
IF: An anonymous customer has reported staining of paraffin embedded endocrine islets (red), nuclear (blue), and ductal cell (green) in adult mouse pancreas. WB: Preliminary experiments in human and mouse brain lysates gave no specific signal but low background. IHC-P: Human cerebral cortex shows staining of some neuronal bodies plus intense axonal staining.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UNC5H3
Long Name
Netrin Receptor UNC5C
Alternate Names
UNC5C
Gene Symbol
UNC5C
UniProt
Additional UNC5H3 Products
Product Documents for UNC5H3/UNC5C Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UNC5H3/UNC5C Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for UNC5H3/UNC5C Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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