Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals | Catalog # NB820-89439
Key Product Details
Species
Applications
Product Summary for Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)
Prepared from several male and female Balb/C mice whole bodies without fur. This protein lysate serves as standard for Western Blot control of human protein studies.
Contents
2 x 0.5 mg per package
Product Specifications
Application Notes
Specificity
Type
Life Stage
Tissue
Protein State
Tissue Condition
Subcellular Fraction
Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Universal Protein
Additional Universal Protein Products
Product Documents for Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)
Certificate of Analysis
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Product Specific Notices for Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)
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Q: I want to test different proteases on a variety of substrates, and thought universal whole tissue lysate as a good choice. Unfortunately it contains a cocktail of protease inhibitors. Is it possible to get some without these inhibitors?
A: We can make the protein without the protease inhibitors, but the minimum quantity for this custom product will be 5 mg. If you do want to obtain this format, please do let us know and we could work on the pricing and its lead time for this custom order.
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Q: Do you know the size of the inhibitors in this cocktail? I am going to denature the proteins, so if they are susceptible to denaturation I can forget about them? Or should I use a spin column to remove low molecular weight compounds?
A: These inhibitors are all < 10 kDa in size, so if you don't want to buy a large quantity, you can definitely use a spin column with a 10 kDa molecular weight cutoff to remove them. I feel that this is the better strategy. This is because, without protease inhibitors, there will be both denaturation and degradation of the lysate protein. If you are performing protease studies, it is better to start with an intact full-length protein.
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Q: I want to test different proteases on a variety of substrates, and thought universal whole tissue lysate as a good choice. Unfortunately it contains a cocktail of protease inhibitors. Is it possible to get some without these inhibitors?
A: We can make the protein without the protease inhibitors, but the minimum quantity for this custom product will be 5 mg. If you do want to obtain this format, please do let us know and we could work on the pricing and its lead time for this custom order.
-
Q: Do you know the size of the inhibitors in this cocktail? I am going to denature the proteins, so if they are susceptible to denaturation I can forget about them? Or should I use a spin column to remove low molecular weight compounds?
A: These inhibitors are all < 10 kDa in size, so if you don't want to buy a large quantity, you can definitely use a spin column with a 10 kDa molecular weight cutoff to remove them. I feel that this is the better strategy. This is because, without protease inhibitors, there will be both denaturation and degradation of the lysate protein. If you are performing protease studies, it is better to start with an intact full-length protein.