URM1 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-469

Novus Biologicals
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Key Product Details

Species Reactivity

Yeast

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all URM1 Primary Antibodies »

Product Specifications

Immunogen

This purified URM1 Antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast URM1 protein. (Uniprot: P40554)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for URM1 Antibody - BSA Free

Western Blot: URM1 Antibody [NB600-469]

Western Blot: URM1 Antibody [NB600-469]

Western Blot: URM1 Antibody [NB600-469] - Western blot using antibody immunized with recombinant yeast URM1 & yeast lysates expressing URM1-GFP fusion protein & other UBL fusion proteins. Panel A: Ponceau total protein staining Panel B: positions of free GFP or GFP- recombinant proteins after reaction with 1:1,000 Anti-GFP, followed by 1:15,000 HRP Donkey-a-Goat IgG MX. Panel C: Specific reaction with URM1 using 1:1,000 IgG fraction of Rabbit-anti- URM1 (Yeast) followed by 1:15,000 HRP Goat-a-Rabbit IgG MX. All primaries diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using 15% gel. Data indicates anti-URM1 is highly specific & does not cross react with other UBLs. Bands in Panel C indicate that URM1 & conjugated URM1 are present in most yeast cell lysates, but at lower levels relative to the URM1-GFP transfected lysate. Chemiluminescence system was used for signal detection (Roche).
N/A: URM1 Antibody [NB600-469]

Applications for URM1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Western Blot

1:500-1:2000
Application Notes
This purified polyclonal antibody reacts with yeast Urm1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: URM1

URM1 acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys),tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at itsC-terminus by MOCS3. The sulfur is then

Long Name

Ubiquitin Related Modifier 1

Alternate Names

C9orf74

Gene Symbol

URM1

UniProt

P40554 (Yeast)

Additional URM1 Products

Product Documents for URM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for URM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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