Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP16 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human ubiquitin specific peptidase 16 using the numbering given in entry NP_006438.1 (GeneID 10600).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP16 Antibody - BSA Free

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for IP at 3 mcg/mg lysate. USP16 was also immunoprecipitated by rabbit anti- USP16 antibody NB100-81639, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for USP16 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP16

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Entrez Gene IDs

10600 (Human)

Gene Symbol

USP16

UniProt

NP_006438.1 (Human)

Additional USP16 Products

Product Documents for USP16 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for USP16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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FAQs

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