Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP20 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 863 and 913 of human ubiquitin specific peptidase 20 (pVHL-interacting deubiquitinating enzyme 2) using the numbering given in entry NP_006667.2 (GeneID 10868).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP20 Antibody - BSA Free

Western Blot: USP20 Antibody [NB100-59837]

Western Blot: USP20 Antibody [NB100-59837]

Western Blot: USP20 Antibody [NB100-59837] - Detection of Mouse USP20/VDU2 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-USP20/VDU2 antibody NB100-59837 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: USP20 Antibody [NB100-59837]

Western Blot: USP20 Antibody [NB100-59837]

Western Blot: USP20 Antibody [NB100-59837] - Detection of Human USP20/VDU2 on HeLa whole cell lysate (5, 15 and 50 microgram for WB; 1mg for IP, 20% of IP loaded) and 293T (T; 50 microgram) using NB100-59837. USP20/VDU2 was also immunoprecipitated by another rabbit anti-USP20/VDU2 antibody.

Applications for USP20 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP20

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 20

Alternate Names

LSFR3A, Ubiquitin Thiolesterase 20, VDU2

Entrez Gene IDs

10868 (Human)

Gene Symbol

USP20

UniProt

NP_006667.2 (Human)

Additional USP20 Products

Product Documents for USP20 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for USP20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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