USP33 Antibody (5B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00023032-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B5

Format

Azide and BSA Free
View all USP33 Primary Antibodies »

Product Specifications

Immunogen

USP33 (NP_055832, 327 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQIL

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasmic

Specificity

USP33 - ubiquitin specific protease 33

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for USP33 Antibody (5B5) - Azide and BSA Free

Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody (M01), clone 5B5.Lane 1: USP33 transfected lysate(103.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01]

Immunocytochemistry/ Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01]

Immunocytochemistry/Immunofluorescence: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01]

Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01]

Immunohistochemistry-Paraffin: USP33 Antibody (5B5) [H00023032-M01] - Analysis of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01]

Western Blot: USP33 Antibody (5B5) [H00023032-M01] - USP33 expression in HeLa ( Cat # L013V1 ).
Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01]

Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01]

Immunoprecipitation: USP33 Antibody (5B5) [H00023032-M01] - Analysis of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: USP33 Antibody (5B5) [H00023032-M01] - Detection limit for recombinant GST tagged USP33 is approximately 0.1ng/ml as a capture antibody.

Applications for USP33 Antibody (5B5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP33

The ubiquitin dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitination, is catalyzed by proteases called deubiquitinating enzymes. USP33 is a deubiquitinating enzyme that binds to the von Hippel-Lindau tumor suppressor protein. It also regulates thyroid hormone activation by deubiquitinating type 2 iodothyronine deiodinase.

Long Name

Ubiquitin carboxyl-terminal hydrolase 33

Alternate Names

hVDU1, KIAA1097, VDU1

Entrez Gene IDs

23032 (Human)

Gene Symbol

USP33

UniProt

NP_055832 (Human)

Additional USP33 Products

Product Documents for USP33 Antibody (5B5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for USP33 Antibody (5B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for USP33 Antibody (5B5) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review USP33 Antibody (5B5) - Azide and BSA Free and earn rewards!

Have you used USP33 Antibody (5B5) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies