VASP [p Ser157] Antibody (2P0U3)

Novus Biologicals | Catalog # NBP3-33585

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2P0U3
View all VASP Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1).

Sequence:
RRVSNA

Modification

p Ser157

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASP [p Ser157] Antibody (2P0U3)

VASP [p Ser157] Antibody (2P0U3)

Western Blot: VASP [p Ser157] Antibody (2P0U3) [NBP3-33585] -

Western Blot: VASP [p Ser157] Antibody (2P0U3) [NBP3-33585] - Western blot analysis of lysates from HEL cells, using VASP Rabbit mAb at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for VASP [p Ser157] Antibody (2P0U3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP [p Ser157] Antibody (2P0U3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VASP [p Ser157] Antibody (2P0U3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Platelet Markers

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