Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all VASP Primary Antibodies »

Product Specifications

Immunogen

The epitope is recognized to a region between residue 330 to 380 of human Vasodilator-stimulated phosphoprotein using the numbering given in entry P50552 (GeneID 7408).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VASP Antibody - BSA Free

Western Blot: VASP Antibody [NBP2-41363]

Western Blot: VASP Antibody [NBP2-41363]

Western Blot: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: VASP Antibody [NBP2-41363]

Western Blot: VASP Antibody [NBP2-41363]

Western Blot: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: VASP Antibody [NBP2-41363]

Immunoprecipitation: VASP Antibody [NBP2-41363]

Immunoprecipitation: VASP Antibody [NBP2-41363] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for IP at 6 ug per reaction. For blotting immunoprecipitated VASP, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: VASP Antibody [NBP2-41363]

Immunoprecipitation: VASP Antibody [NBP2-41363]

Immunoprecipitation: VASP Antibody [NBP2-41363] - Detection of human VASP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VASP antibody NBP2-41363 used for IP at 6 ug per reaction. VASP was also immunoprecipitated by another rabbit anti-VASP antibody. For blotting immunoprecipitated VASP, NBP2-41363 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for VASP Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

UniProt

NP_077292.1 (Human)

Additional VASP Products

Product Documents for VASP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for VASP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Platelet Markers

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