Vav-1 Antibody (2E11) - BSA Free

Novus Biologicals | Catalog # NBP2-37469

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E11

Format

BSA Free
View all Vav-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Vav-1 (AA: 121-324) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

98.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Vav-1 Antibody (2E11) - BSA Free (NBP2-37469) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Vav-1 Antibody (2E11) - BSA Free

Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mouse mAb against Jurkat (1) cell lysate.
Flow Cytometry: Vav-1 Antibody (2E11) - BSA Free [NBP2-37469]

Flow Cytometry: Vav-1 Antibody (2E11) - BSA Free [NBP2-37469]

Flow Cytometry: Vav-1 Antibody (2E11) [NBP2-37469] - Flow cytometric analysis of HeLa cells using VAV1 mouse mAb (green) and negative control (red).
Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mAb against human VAV1 recombinant protein. (Expected MW is 49.3 kDa)
Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11)BSA Free [NBP2-37469]

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mAb against HEK293 (1) and VAV1 (AA: 121-324)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: Vav-1 Antibody (2E11) - BSA Free [NBP2-37469]

ELISA: Vav-1 Antibody (2E11) - BSA Free [NBP2-37469]

ELISA: Vav-1 Antibody (2E11) [NBP2-37469] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Vav-1 Antibody (2E11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:250
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vav-1

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Alternate Names

vav-T, Vav1

Gene Symbol

VAV1

Additional Vav-1 Products

Product Documents for Vav-1 Antibody (2E11) - BSA Free

Certificate of Analysis

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Product Specific Notices for Vav-1 Antibody (2E11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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