VAV3 Antibody

Novus Biologicals | Catalog # NBP3-23274

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all VAV3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the Dbl homology domain of human Vav3. This sequence has high homology with similar regions in rat and mouse Vav3, and has low homology to other Vav family members. [UniProt# Q9UKW4]

Specificity

The antibody detects a 98 kDa protein corresponding to the molecular mass of Vav3 on SDS-PAGE immunoblots of human Jurkat cells. The antibody also strongly detects an unidentified protein at 120 kDa in Jurkat and K562.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for VAV3 Antibody

VAV3 Antibody

Western Blot: VAV3 Antibody [NBP3-23274] -

Western Blot: VAV3 Antibody [NBP3-23274] - Western blot of human Jurkat (lanes 1 & 4), HUVEC (lanes 2 & 5), and A431 (lanes 3 & 6) cells. The blots were probed with anti-VAV2 (a.a. 309-322) at a dilution of 1:500 (lanes 1-3) and anti-VAV3 (a.a. 293-305) at 1:500 (lanes 4-6).

Applications for VAV3 Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VAV3

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). Vav3 is expressed in most hematopoietic cells and non-hematopoietic tissues except ovary and skeletal muscles. T-cell stimulated and tyrosine phosphorylated Vav3 acts as an exchange factor for GTP-binding proteins RhoA, RhoG, and Rac1 (2). Also, tyrosine phosphorylated Vav3 mediates RTK signalizing, specifically IR, IGFR, and EGFR-mediated signaling pathways. Vav3 has been linked to as a factor for cell morphology modulation and an inducer for cell transformation (3).

Alternate Names

FLJ40431, guanine nucleotide exchange factor VAV3, vav 3 guanine nucleotide exchange factor, vav 3 oncogene, VAV-3

Gene Symbol

VAV3

Additional VAV3 Products

Product Documents for VAV3 Antibody

Certificate of Analysis

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Product Specific Notices for VAV3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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