![Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49940]](https://resources.rndsystems.com/images/products/VCIP135-VCPIP1-Antibody-Western-Blot-NBP1-49940-img0003.jpg "Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49940]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1172 and 1222 of human Valosin-Containing Protein (p97)/p47 Complex-Interacting Protein p135 using the numbering given in entry NP_079330.2 (GeneID 80124).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VCIP135/VCPIP1 Antibody
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49940]
Western Blot: VCIP135/VCPIP1 Antibody [NBP1-49940] - VCPIP1 Antibody [NBP1-49940] - Detection of Human and Mouse VCIP135 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-VCIP135 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. VCIP135 was also immunoprecipitated by rabbit anti-VCIP135 antibody which recognizes an upstream epitope. For blotting immunoprecipitated VCIP135 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 second (B).Applications for VCIP135/VCPIP1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: VCIP135/VCPIP1
Long Name
Valosin-Containing Protein P97/P47 Complex-Interacting Protein p135
Alternate Names
DUBA3, VCPIP1
Gene Symbol
VCPIP1
UniProt
Additional VCIP135/VCPIP1 Products
Product Documents for VCIP135/VCPIP1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VCIP135/VCPIP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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