VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

Inhibits photobleaching of fluorescent dyes and fluorescent proteins

Ideal refractive index (1.45)

Ready-to-use

No warming necessary

Can be stored without sealing for long term analysis

Hardening formulation

DAPI counterstain

VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.