Viral EBOV GP Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB9016AFP680

R&D Systems

Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 993408
View all EBOV GP Primary Antibodies »

Product Specifications

Specificity

Detects EBOV GP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Viral EBOV GP Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).

References

  1. Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
  2. de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
  3. Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
  4. Volchkov, V.E. et al. (1998) Virology 245:110.
  5. Sanchez, A. et al. (1998) J. Virol. 72:6442.
  6. Dolnik, O. et al. (2004) EMBO J. 23:2175.
  7. Takada, A. et al. (2004) J. Virol. 78:2943.
  8. Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
  9. Simmons, G. et al. (2003) Virology 305:115.
  10. Schornberg, K. et al. (2006) J. Virol. 80:4174.
  11. Chandran, K. et al. (2005) Science 308:1643.
  12. Cote, M. et al. (2011) Nature 477:344.

Long Name

Ebola Virus Glycoprotein

Alternate Names

bovGP

Entrez Gene IDs

911829 (Viral)

Gene Symbol

GP

UniProt

Q05320

Additional EBOV GP Products

Product Documents for Viral EBOV GP Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Viral EBOV GP Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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