Viral EBOV GP Antibody Summary
Accession # Q05320
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of EBOV GP by Western Blot. Western blot shows Recombinant EBOV GP. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral EBOV GP Monoclonal Antibody (Catalog # MAB9016) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EBOV GP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBOV GP
The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).
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- Volchkov, V.E. et al. (1998) Virology 245:110.
- Sanchez, A. et al. (1998) J. Virol. 72:6442.
- Dolnik, O. et al. (2004) EMBO J. 23:2175.
- Takada, A. et al. (2004) J. Virol. 78:2943.
- Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
- Simmons, G. et al. (2003) Virology 305:115.
- Schornberg, K. et al. (2006) J. Virol. 80:4174.
- Chandran, K. et al. (2005) Science 308:1643.
- Cote, M. et al. (2011) Nature 477:344.
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