Key Product Details

Species Reactivity

Viral

Applications

Western Blot

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Mouse IgG2A Clone # 993408
View all EBOV GP Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant Viral EBOV GP
Ile33-Arg501
Accession # Q05320

Specificity

Detects EBOV GP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for EBOV GP Antibody (993408) [CoraFluor™ 1]

EBOV GP Antibody (993408) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for EBOV GP Antibody (993408) [CoraFluor™ 1]

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: EBOV GP

The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses. It is processed into GP1 and GP2 fragments which remain disulfide linked. GP1 is entirely extracellular while GP2 is a transmembrane protein. GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2. GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR. Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane.

Long Name

Ebola Virus Glycoprotein

Alternate Names

bovGP

Additional EBOV GP Products

Product Documents for EBOV GP Antibody (993408) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EBOV GP Antibody (993408) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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