Canine Vitamin A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60190

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

2.336 ng/mL

Assay Range

3.12 ng/mL - 50 ng/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Canine

Specificity

This assay has high sensitivity and excellent specificity for detection of dog VA. No significant cross-reactivity or interference between dog VA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Canine Vitamin A ELISA Kit (Colorimetric)

ELISA: Canine Vitamin A ELISA Kit (Colorimetric) [NBP2-60190]

ELISA: Canine Vitamin A ELISA Kit (Colorimetric) [NBP2-60190]

ELISA: Canine Vitamin A ELISA Kit (Colorimetric) [NBP2-60190] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Canine Vitamin A ELISA Kit (Colorimetric)

Canine Vitamin A ELISA Kit (Colorimetric) [NBP2-60190] -

Canine Vitamin A ELISA Kit (Colorimetric) [NBP2-60190] - Standard Curve Reference

Kit Contents for Canine Vitamin A ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin A

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VA antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Additional Vitamin A Products

Product Documents for Canine Vitamin A ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Canine Vitamin A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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