Human Vitamin A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60191

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.467 ng/mL

Assay Range

0.625 ng/mL - 10 ng/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human VA. No significant cross-reactivity or interference between human VA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human Vitamin A ELISA Kit (Colorimetric)

Human Vitamin A ELISA Kit (Colorimetric)

Human Vitamin A ELISA Kit (Colorimetric) [NBP2-60191] -

Human Vitamin A ELISA Kit (Colorimetric) [NBP2-60191] - Standard Curve Reference

Kit Contents for Human Vitamin A ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin A

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Additional Vitamin A Products

Product Documents for Human Vitamin A ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Vitamin A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human Vitamin A ELISA Kit (Colorimetric)

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