Mouse Vitamin A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60192

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

140.159 pg/mL

Assay Range

187.5 pg/mL - 3000 pg/mL
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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse VA. No significant cross-reactivity or interference between mouse VA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Mouse Vitamin A ELISA Kit (Colorimetric)

Mouse Vitamin A ELISA Kit (Colorimetric)

Mouse Vitamin A ELISA Kit (Colorimetric) [NBP2-60192] -

Mouse Vitamin A ELISA Kit (Colorimetric) [NBP2-60192] - Standard Curve Reference

Kit Contents for Mouse Vitamin A ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Vitamin A

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Additional Vitamin A Products

Product Documents for Mouse Vitamin A ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse Vitamin A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse Vitamin A ELISA Kit (Colorimetric)

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