Q: We tested both NBP1-49669 and R-079-100 by WB, but failed to obtain signal. May we ask your suggestion for positive control? We found NBL1-17341 may be an option for R-079-100, but is there any other lysate which can be suggested and is easier to get?

A: In our catalog, NBP2-10877 as well as NBP2-05057 are the products that are suggested as lysates for ZO-1 tight junction protein antibody (NBP1-49669). You already know NBL1-17341 may be an option for VRL1 Antibody (R-079-100). I do not see any other products in our collection that will go along ZO-1 tight junction protein antibody (NBP1-49669) and VRL1 antibody (R-079-100).

VRL1 Overexpression Lysate

Novus Biologicals | Catalog # NBL1-17341

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for VRL1 Overexpression Lysate

VRL1 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC202821

Accession#: NM_016113

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

85.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for VRL1 Overexpression Lysate

Western Blot: VRL1 Overexpression Lysate [NBL1-17341]

Western Blot: VRL1 Overexpression Lysate (Adult Normal) [NBL1-17341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VRL1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: VRL1

This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Transient receptor potential cation channel subfamily V member 2

Alternate Names

OTRPC2, TrpV2, VRL, VRL-1

Gene Symbol

TRPV2

Additional VRL1 Products

Product Documents for VRL1 Overexpression Lysate

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for VRL1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customer Reviews for VRL1 Overexpression Lysate

There are currently no reviews for this product. Be the first to review VRL1 Overexpression Lysate and earn rewards!

Have you used VRL1 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image