WDR68 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-92589
![Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Immunohistochemistry-Paraffin-NBP1-92589-img0008.jpg "Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Drosophila
Cited:
Insect - Drosophila
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PCTPVARLNNHRACVNGIAWAPHSSCHICTAADDHQALIWDIQQMPRAIEDPILAYTAEGEINNVQWASTQPDWIAICYNNC
Reactivity Notes
Drosophila reactivity reported in scientific literature (PMID: 23955303).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for WDR68 Antibody - BSA Free
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589] - Staining of human cerebral cortex, kidney, liver and testis using Anti-DCAF7 antibody NBP1-92589 (A) shows similar protein distribution across tissues to independent antibody NBP1-92590 (B).![Western Blot: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Western-Blot-NBP1-92589-img0007.jpg "Western Blot: WDR68 Antibody [NBP1-92589]")
Western Blot: WDR68 Antibody [NBP1-92589]
Western Blot: WDR68 Antibody [NBP1-92589] - Analysis in human cell line SH-SY5Y.![Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Immunohistochemistry-Paraffin-NBP1-92589-img0011.jpg "Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]")
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589] - Staining of human testis.![Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Immunohistochemistry-Paraffin-NBP1-92589-img0009.jpg "Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]")
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589] - Staining of human liver.![Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Western-Blot-NBP1-92589-img0010.jpg "Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]")
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589] - Staining of human cerebral cortex.![Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]](https://resources.rndsystems.com/images/products/WDR68-Antibody-Immunohistochemistry-Paraffin-NBP1-92589-img0012.jpg "Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]")
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589]
Immunohistochemistry-Paraffin: WDR68 Antibody [NBP1-92589] - Staining of human kidney.![WDR68 Antibody - BSA Free Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-29520251655915.jpg "Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.![WDR68 Antibody - BSA Free Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-295202516264518.jpg "Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.![WDR68 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-295202516255815.jpg "Immunocytochemistry/ Immunofluorescence: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunocytochemistry/ Immunofluorescence: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & cytosol.![WDR68 Antibody - BSA Free Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-295202516321324.jpg "Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.![WDR68 Antibody - BSA Free Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-30520251043812.jpg "Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.![WDR68 Antibody - BSA Free Western Blot: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-29520251652419.jpg "Western Blot: WDR68 Antibody - BSA Free [NBP1-92589]")
Western Blot: WDR68 Antibody - BSA Free [NBP1-92589]
Analysis in human cell line SH-SY5Y.![WDR68 Antibody - BSA Free Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]](https://resources.rndsystems.com/images/products/nbp1-92589_rabbit-polyclonal-wdr68-antibody-29520251631223.jpg "Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]")
Immunohistochemistry: WDR68 Antibody - BSA Free [NBP1-92589]
Staining of human cerebral cortex).Applications for WDR68 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: WDR68
Alternate Names
DDB1 and CUL4 associated factor 7, DDB1- and CUL4-associated factor 7, HAN11AN11, human anthocyanin, seven-WD-repeat protein of the AN11 family-1, SWAN-1, WD repeat domain 68, WD repeat-containing protein 68, WD repeat-containing protein An11 homolog, WDR68, WD-repeat protein
Entrez Gene IDs
10238 (Human)
Gene Symbol
DCAF7
UniProt
Additional WDR68 Products
Product Documents for WDR68 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WDR68 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for WDR68 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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