WDR79 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92592

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all WDR79 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GSLSEEEANGPELGSGKAMEDTSGEPAAEDEGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVEYAEMVPVLRMVEGDTIYDYC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%). Human reactivity reported in scientific literature (PMID: 26426684).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR79 Antibody - BSA Free

Western Blot: WDR79 Antibody [NBP1-92592]

Western Blot: WDR79 Antibody [NBP1-92592]

Western Blot: WDR79 Antibody [NBP1-92592] - Analysis in control (vector only transfected HEK293T lysate) and WRAP53 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: WDR79 Antibody [NBP1-92592]

Immunocytochemistry/ Immunofluorescence: WDR79 Antibody [NBP1-92592]

Immunocytochemistry/Immunofluorescence: WDR79 Antibody [NBP1-92592] - Staining of human cell line A-431 shows localization to nuclear bodies.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human skin shows weak nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human fallopian tube, kidney, liver and skin using Anti-WRAP53 antibody NBP1-92592 (A) shows similar protein distribution across tissues to independent antibody NBP1-92593 (B).
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592]

Immunohistochemistry-Paraffin: WDR79 Antibody [NBP1-92592] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for WDR79 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.Use in Western blot reported in scientific literature (PMID 28347242).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WDR79

WD-repeat domain 79 (WDR79) contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.

Alternate Names

FLJ10385, TCAB1WDR79telomerase Cajal body protein 1, WD repeat containing, antisense to TP53, WD repeat domain 79, WD repeat-containing protein 79, WD40 protein Wrap53, WD40 repeat-containing protein encoding RNA antisense to p53, WD-encoding RNA antisense to p53

Gene Symbol

WRAP53

Additional WDR79 Products

Product Documents for WDR79 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for WDR79 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for WDR79 Antibody - BSA Free

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Protocols

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