Chemical Name: 4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide
Biological ActivityWnt-C59 is a highly potent inhibitor of MBOAT (membrane-bound O-acyltranferase) family member Porcupine (PORCN) (IC50 = 74 pM) that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59 treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2+/COUP-TF1- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. Cell permeable and orally bioavailable.
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Chemically defined generation of human cardiomyocytes.
Burridge et al.
Pharmacological inhibition of the Wnt acyltransferase PORCN prevents growth of WNT-driven mammary cancer.
Proffitt et al.
Cancer Res., 2013;73:502
Adult interfollicular tumour-initiating cells are reprogrammed into an embryonic hair follicle progenitor-like fate during basal cell carcinoma initiation.
Youssef et al.
Nat. Cell Biol., 2012;14:1282
WNT10B/β-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer.
Wend et al.
EMBO Mol.Med., 2013;5:264
Citations for Wnt-C59
The citations listed below are publications that use Tocris products. Selected citations for Wnt-C59 include:
14 Citations: Showing 1 - 10
A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees.
Authors: Ward and Gilad
Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.
Authors: Liang Et al.
Stem Cells 2019;38:352
Capacitation of human na�ve pluripotent stem cells for multi-lineage differentiation.
Authors: Rostovskaya Et al.
Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES.
Authors: Pfeiffer Et al.
Nat Commun 2018;9:440
An Ultrasensitive Calcium Reporter System via CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
Authors: Jiang Et al.
Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation.
Authors: Sharma Et al.
Sci Rep 2018;8:6618
A Comparative Assessment of Human and Chimpanzee iPSC-derived Cardiomyocytes with Primary Heart Tissues.
Authors: Pavlovic Et al.
Sci Rep 2018;8:15312
A promoter interaction map for cardiovascular disease genetics.
Authors: Montefiori Et al.
Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
Authors: Shafa Et al.
Front Med (Lausanne) 2018;5:69
Iron deficiency impairs contractility of human cardiomyocytes through decreased mitochondrial function.
Authors: Hoes Et al.
Eur J Heart Fail 2018;20:910
Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling.
Authors: Piccini Et al.
Front Physiol 2017;8:705
Cardiac Subtype-Specific Modeling of Kv1.5 Ion Channel Deficiency Using Human Pluripotent Stem Cells.
Authors: Marczenke Et al.
Front Physiol 2017;8:469
A Novel Role for the BMP Antagonist Noggin in Sensitizing Cells to Non-canonical Wnt-5a/Ror2/Disheveled Pathway Activation.
Authors: Bernatik Et al.
Front Cell Dev Biol 2017;5:47
Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development.
Authors: Nigmatullina Et al.
EMBO J 2017;36:869
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