Home / PORCN Inhibitors / Wnt-C59

Wnt-C59

Catalog #: 5148
14 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5148/10
5148/50
Wnt-C59 | CAS No. 1243243-89-1 | PORCN Inhibitors
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Description: Highly potent PORCN inhibitor

Chemical Name: 4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide

Purity: ≥99%

Product Details
Citations (14)
Reviews (1)
Biological Activity Technical Data Background Product Datasheets Calculators

Biological Activity

Wnt-C59 is a highly potent inhibitor of MBOAT (membrane-bound O-acyltranferase) family member Porcupine (PORCN) (IC50 = 74 pM) that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59 treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2+/COUP-TF1- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. Cell permeable and orally bioavailable.

Technical Data

M.Wt:
379.45
Formula:
C25H21N3O
Solubility:
Soluble to 20 mM in DMSO and to 20 mM in ethanol
Purity:
≥99%
Storage:
Store at -20°C
CAS No:
1243243-89-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for Wnt-C59

The citations listed below are publications that use Tocris products. Selected citations for Wnt-C59 include:

14 Citations: Showing 1 - 10

  1. A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees.
    Authors: Ward and Gilad
    Elife  2019;8
  2. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.
    Authors: Liang Et al.
    Stem Cells  2019;38:352
  3. Capacitation of human na�ve pluripotent stem cells for multi-lineage differentiation.
    Authors: Rostovskaya Et al.
    Development  2019;146
  4. Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES.
    Authors: Pfeiffer Et al.
    Nat Commun  2018;9:440
  5. An Ultrasensitive Calcium Reporter System via CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
    Authors: Jiang Et al.
    iScience  2018;9:27
  6. Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation.
    Authors: Sharma Et al.
    Sci Rep  2018;8:6618
  7. A Comparative Assessment of Human and Chimpanzee iPSC-derived Cardiomyocytes with Primary Heart Tissues.
    Authors: Pavlovic Et al.
    Sci Rep  2018;8:15312
  8. A promoter interaction map for cardiovascular disease genetics.
    Authors: Montefiori Et al.
    Elife  2018;7
  9. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
    Authors: Shafa Et al.
    Front Med (Lausanne)  2018;5:69
  10. Iron deficiency impairs contractility of human cardiomyocytes through decreased mitochondrial function.
    Authors: Hoes Et al.
    Eur J Heart Fail  2018;20:910

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iPSC differentiation
By Anonymous on 10/28/2021
Species: Human

Used to differentiate human iPSC into cardiac cells. dose of 2uM was used

iPSC differentiation 5148
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