Wnt-C59

Tocris Bioscience | Catalog # 5148

Highly potent PORCN inhibitor
Tocris Bioscience

Key Product Details

Description

Highly potent PORCN inhibitor
View all PORCN Inhibitors »

Product Description

Wnt-C59 is a highly potent inhibitor of the MBOAT (membrane-bound O-acyltranferase) family member, Porcupine (PORCN) (IC50 = 74 pM), that mediates WNT palmitoylation and secretion. Wnt-C59 potently inhibits the processing of both canonical (1, 2, 3a, 6, 7b, 8a, 9a, 9b, 10) and non-canonical (4, 5a, 11, 16) Wnt subtypes. Wnt-C59 blocks progression of mammary tumors in MMTV-WNT1 transgenic mice and downregulates Wnt/β-catenin target genes. Wnt-C59-treated tumors show a decrease in β-catenin, CyclinD1 and c-Myc. Wnt-C59 induces cardiomyocyte differentiation from human iPSCs following culture with CHIR 99021 (Cat. No. 4423). Wnt-C59 efficiently induces neural differentiation of CTIP2+/COUP-TF1- cells from PSCs in culture. When grafted into the cortex of adult mice, Wnt-C59-treated cells develop abundant axonal fiber extensions toward the spinal cord. The compound has also been used in protocols to generate β cells from human PSCs. Cell permeable and orally bioavailable.

Product Specifications for Wnt-C59

Molecular Weight

379.45

Formula

C25H21N3O

Storage

Store at -20°C

Purity

≥99% (HPLC)

Chemical Name

4-(2-Methyl-4-pyridinyl)-N-[4-(3-pyridinyl)phenyl]benzeneacetamide

CAS Number

1243243-89-1

PubChem ID

57519544

InChI Key

KHZOJCQBHJUJFY-UHFFFAOYSA-N

SMILES

O=C(CC3=CC=C(C4=CC=NC(C)=C4)C=C3)NC1=CC=C(C2=CC=CN=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.59 20
Ethanol 7.59 20

Preparing Stock Solutions for Wnt-C59

The following data is based on the product molecular weight 379.45.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.18 mL 65.88 mL 131.77 mL
1 mM 2.64 mL 13.18 mL 26.35 mL
2 mM 1.32 mL 6.59 mL 13.18 mL
10 mM 0.26 mL 1.32 mL 2.64 mL

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Product Documents for Wnt-C59

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Wnt-C59

For research use only

Customer Reviews for Wnt-C59 (2)

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  • iPSC differentiation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Human iPSC
    Verified Customer | Posted 10/28/2021
    Used to differentiate human iPSC into cardiac cells. dose of 2uM was used
    Wnt-C59 5148
  • The role of Wnt signaling in chemotherapy-induced apoptosis.
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 06/10/2021
    Pancreatic cancer cells were incubated for pretreated for 2 hours with WNT-C59 followed by conventional chemotherapeutics. Wnt inhibition had an additive effect on cancer cell’s viability upon 3 days of treatment.

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