Home / GSK-3 Inhibitors / CHIR 99021

CHIR 99021

Catalog #: 4423
409 Citations 2 Reviews Datasheet / COA / SDS
GMP Version Available: TB4423-GMP
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4423/50
CHIR 99021 | CAS No. 252917-06-9 | GSK-3 Inhibitors
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Description: Highly selective GSK-3 inhibitor; acts as Wnt activator
Alternative Names: CHIR99021, CT99021

Chemical Name: 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile

Purity: ≥98%

Product Details
Citations (409)
Supplemental Products
Reviews (2)
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

CHIR 99021 is a potent and highly selective inhibitor of glycogen synthase kinase 3 (GSK-3) (IC50 values are 6.7 and 10 nM for GSK-3β and GSK-3α respectively). Acts as Wnt activator. Exhibits >500-fold selectivity for GSK-3 over closely related kinases; also displays >800-fold selectivity against 45 additional enzymes and receptors. In combination with the irreversible inhibitor of LSD1 (Cat. No. 3852), CHIR 99021 enables reprogramming of mouse embryonic fibroblasts, transduced by Oct4 and Klf4 only, into iPSCs. Enhances mouse and human ESC self-renewal when used in combination with PD 0325901 (Cat. No. 4192). It promotes differentiation of hPSCs in cardiomyocytes and allows formation of extended pluripotent stem (EPS) cells in combination with (S)-(+)-Dimethindene maleate (Cat. No. 1425), tetracycline antibiotic (Cat. No. 3268) and human leukemia inhibitory factor. Also used in a protocol to generate brain organoids from human iPSCs.

CHIR 99021 synthesized to cGMP guidelines also available.

For more information on how to use CHIR 99021, see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts.

Scientific Data

N/A Cardiomyocyte derived from human induced pluripotent stem cells (hiPSCs) using CHIR 99021. View Larger

Cardiomyocyte derived from human induced pluripotent stem cells (hiPSCs) using CHIR 99021. Cells cultured in Media supplemented with Activin A (Catalog # 338-AC, R&D Systems), N21-MAX Insulin Free (Catalog # AR010, R&D Systems), BMP-4 (Catalog # 314-BP, R&D Systems), bFGF (Catalog # 233-FB, R&D Systems) and DKK-1 (Catalog # 5439-DK, R&D Systems). Stained for F-Actin (Phalloidin, green), Troponin T (red, Human Troponin T (Cardiac) Antibody, Catalog # MAB1874, R&D Systems) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

External Portal Information

Chemicalprobes.org is a portal that offers independent guidance on the selection and/or application of small molecules for research. The use of CHIR-99021 is reviewed on the chemical probes website.

Technical Data

M.Wt:
465.34
Formula:
C22H18Cl2N8
Solubility:
Soluble to 20 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
252917-06-9

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.
    Xia Y, Sancho-Martinez I, Nivet E et al.
    Nat Protoc.
  2. 3D mouse embryonic stem cell culture for generating inner ear organoids.
    Koehler KR, Hashino E.
    Nat Protoc
  3. AKT kinase activity is required for Li to modulate mood-related behaviors in mice.
    Pan et al.
    Neuropsychopharmacology, 2011;36:1397
  4. Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes self-renewal of embyronic stem cells from refractory mouse strains.
    Ye et al.
    PLoS One, 2012;7:e35892
  5. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.
    Li et al.
    Stem Cells, 2009;27:2992
  6. Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency.
    Yang et al.
    Cell., 2017;169:243
  7. In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection.
    Bartfeld et al.
    Gastroenterology, 2015;148:126
  8. SnapShot: Growing Organoids from Stem Cells.
    Sato et al.
    Cell, 2015;161:1700
  9. Establishment of gastrointestinal epithelial organoids
    Mahe et al.
    Curr.Protoc. Mouse Biol., 2014;3:217
  10. In vitro generation of human pluripotent stem cell derived lung organoids.
    Dye et al.
    Elife, 2015;
  11. Human blood vessel organoids as a model of diabetic vasculopathy.
    Wimmer et al.
    Nature, 2019;565:505
  12. 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts.
    Noor et al.
    Adv.Sci.(Weinh), 2019;6:1900344
  13. Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells.
    Hoang et al.
    Nat.Protoc., 2018;13:723

Product Datasheets

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Citations for CHIR 99021

The citations listed below are publications that use Tocris products. Selected citations for CHIR 99021 include:

409 Citations: Showing 1 - 10

  1. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.
    Authors: Jing Et al.
    NPJ Regen Med  2023;8:26
  2. Systematic discovery of transcription factors that improve hPSC-derived cardiomyocyte maturation via temporal analysis of bioengineered cardiac tissues.
    Authors: Aditya Et al.
    APL Bioeng  2023;7:026109
  3. Spatial multiomics map of trophoblast development in early pregnancy.
    Authors: Qian Et al.
    Nature  2023;616:143-151
  4. Engineered cardiac tissue model of restrictive cardiomyopathy for drug discovery.
    Authors: Yulia V Et al.
    Cell Rep Med  2023;4:100976
  5. A Robust Pipeline for the Multi-Stage Accelerated Differentiation of Functional 3D Cortical Organoids from Human Pluripotent Stem Cells.
    Authors: Mustafa Et al.
    Curr Protoc  2023;3:e641
  6. Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imaging.
    Authors: Stefan Et al.
    Cell Rep Methods  2023;3:100440
  7. Genetic dissection of the pluripotent proteome through multi-omics data integration.
    Authors: Steven P Et al.
    Cell Genom  2023;3:100283
  8. Automated high-content imaging in iPSC-derived neuronal progenitors.
    Authors: Serena Et al.
    SLAS Discov  2023;28:42-51
  9. Generation of dorsoventral human spinal cord organoids via functionalizing composite scaffold for drug testing.
    Authors: Bo Et al.
    iScience  2023;26:105898
  10. Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation.
    Authors: Sara Et al.
    Nat Cell Biol  2023;25:481-492
  11. Long-Term SMN- and Ncald-ASO Combinatorial Therapy in SMA Mice and NCALD-ASO Treatment in hiPSC-Derived Motor Neurons Show Protective Effects.
    Authors: C Frank Et al.
    Int J Mol Sci  2023;24
  12. Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.
    Authors: Dmitry Et al.
    Int J Mol Sci  2023;24
  13. Pump-less, recirculating organ-on-a-chip (rOoC) platform.
    Authors: Stefan Et al.
    Lab Chip  2023;23:591-608
  14. FUS-ALS hiPSC-derived astrocytes impair human motor units through both gain-of-toxicity and loss-of-support mechanisms.
    Authors: Ludo Et al.
    Mol Neurodegener  2023;18:5
  15. Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds.
    Authors: Laween Et al.
    Nat Protoc  2023;18:108-135
  16. An in vivo neuroimmune organoid model to study human microglia phenotypes.
    Authors: Schafer Et al.
    Cell  2023;186:2111
  17. A pesticide and iPSC dopaminergic neuron screen identifies and classifies Parkinson-relevant pesticides.
    Authors: Tim Et al.
    Nat Commun  2023;14:2803
  18. Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids.
    Authors: Jie Et al.
    Bio Protoc  2023;13:e4657
  19. Axon guidance genes modulate neurotoxicity of ALS-associated UBQLN2.
    Authors: Mark Et al.
    Elife  2023;12
  20. Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells.
    Authors: Alessandro Et al.
    Cells  2023;12
  21. Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models.
    Authors: Alessandra Et al.
    Front Cell Dev Biol  2023;11:1111684
  22. A distal lung organoid model to study interstitial lung disease, viral infection and human lung development.
    Authors: Nadine Et al.
    Nat Protoc  2023;
  23. In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice
    Authors: Bouffi Et al.
    Nat.Biotechnol.  2023;
  24. Ti3 C2 Tx MXene Composite 3D Hydrogel Potentiates mTOR Signaling to Promote the Generation of Functional Hair Cells in Cochlea Organoids.
    Authors: Chen Et al.
    Adv Sci (Weinh)  2022;9:e2203557
  25. Transcription Factor Hb9 Is Expressed in Glial Cell Lineages in the Developing Mouse Spinal Cord.
    Authors: Diego Et al.
    Eneuro  2022;9
  26. Engineering a living cardiac pump on a chip using high-precision fabrication.
    Authors: Kamil Et al.
    Sci Adv  2022;8:eabm3791
  27. Transplanted organoids empower human preclinical assessment of drug candidate for the clinic.
    Authors: Michael Et al.
    Sci Adv  2022;8:eabj5633
  28. Reengineering Ponatinib to Minimize Cardiovascular Toxicity.
    Authors: Wenqi Et al.
    Cancer Res  2022;82:2777-2791
  29. JAK inhibitor blocks COVID-19 cytokine-induced JAK/STAT/APOL1 signaling in glomerular cells and podocytopathy in human kidney organoids.
    Authors: Guojie Et al.
    JCI Insight  2022;7
  30. The antimicrobial peptide S100A8/A9 produced by airway epithelium functions as a potent and direct regulator of macrophage phenotype and function.
    Authors: Stefan Et al.
    Eur Respir J  2022;59
  31. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster.
    Authors: Jorge Et al.
    Dev Cell  2022;57:1922-1936.e9
  32. IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.
    Authors: Jay K Et al.
    Immunity  2022;55:237-253.e8
  33. Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response.
    Authors: Yue Et al.
    Redox Biol  2022;50:102248
  34. Organoids capture tissue-specific innate lymphoid cell development in mice and humans.
    Authors: Ludovic Et al.
    Cell Rep  2022;40:111281
  35. STK25 inhibits PKA signaling by phosphorylating PRKAR1A.
    Authors: Gordana Et al.
    Cell Rep  2022;40:111203
  36. In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion.
    Authors: Sandeep Et al.
    Cell Rep  2022;40:111119
  37. An optimized chromatin immunoprecipitation protocol using Staph-seq for analyzing genome-wide protein-DNA interactions.
    Authors: Xi Et al.
    STAR Protoc  2022;3:101918
  38. Generation of human elongating multi-lineage organized cardiac gastruloids.
    Authors: Janet L Et al.
    STAR Protoc  2022;3:101898
  39. Protocols to culture and harvest hepatic tumor organoids for metabolic assays.
    Authors: Stephanie Et al.
    STAR Protoc  2022;3:101597
  40. Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.
    Authors: Aleksandra Et al.
    STAR Protoc  2022;3:101447
  41. Primary culture of tracheal aspirate-derived human airway basal stem cells.
    Authors: Xingbin Et al.
    STAR Protoc  2022;3:101390
  42. Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.
    Authors: Mustafa Et al.
    Curr Protoc  2022;2:e568
  43. Three-Dimensional Vessels-on-a-Chip Based on hiPSC-derived Vascular Endothelial and Smooth Muscle Cells.
    Authors: Marc Et al.
    Curr Protoc  2022;2:e564
  44. Derivation of nociceptive sensory neurons from hiPSCs with early patterning and temporally controlled NEUROG2 overexpression.
    Authors: Emmanouil Et al.
    Cell Rep Methods  2022;2:100341
  45. Development of high-affinity nanobodies specific for NaV1.4 and NaV1.5 voltage-gated sodium channel isoforms.
    Authors: Gordon F Et al.
    J Biol Chem  2022;298:101763
  46. Human induced pluripotent stem cells integrate, create synapses and extend long axons after spinal cord injury.
    Authors: James R Et al.
    J Cell Mol Med  2022;26:1932-1942
  47. Human fetal membrane-mesenchymal stromal cells generate functional spinal motor neurons in vitro.
    Authors: Simone Et al.
    iScience  2022;25:105197
  48. Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes.
    Authors: Kyung-Sun Et al.
    iScience  2022;25:105150
  49. A combined human gastruloid model of cardiogenesis and neurogenesis.
    Authors: Janet L Et al.
    iScience  2022;25:104486
  50. Identification of metabolic pathways underlying FGF1 and CHIR99021-mediated cardioprotection.
    Authors: Fan Et al.
    iScience  2022;25:104447
  51. CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells.
    Authors: Yoshihiro Et al.
    iScience  2022;25:104314
  52. Single-cell atlas of the aging mouse colon.
    Authors: Jing Et al.
    iScience  2022;25:104202
  53. Metabolomic profiling of human pluripotent stem cell differentiation into lung progenitors.
    Authors: Martin Et al.
    iScience  2022;25:103797
  54. The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A.
    Authors: Sagrario Et al.
    Nat Cell Biol  2022;24:1528-1540
  55. Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells.
    Authors: Bruce Et al.
    Nat Cell Biol  2022;24:10-23
  56. In Vitro Generation of Heart Field Specific Cardiomyocytes.
    Authors: Reza Et al.
    Methods Mol Biol  2022;2429:257-267
  57. Differentiation of iPS-Cells into Peripheral Sensory Neurons.
    Authors: Angelika Et al.
    Methods Mol Biol  2022;2429:175-188
  58. Human Pluripotent Stem Cell-Derived Alveolar Organoid with Macrophages.
    Authors: Young-Woock Et al.
    Int J Mol Sci  2022;23
  59. Engineering neurovascular organoids with 3D printed microfluidic chips.
    Authors: Catherine Et al.
    Lab Chip  2022;22:1615-1629
  60. LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells.
    Authors: Chong-Shan Et al.
    Autophagy  2022;18:204-222
  61. Autophagy modulates cell fate decisions during lineage commitment.
    Authors: Elisabeth Et al.
    Autophagy  2022;18:1915-1931
  62. A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication.
    Authors: Chengkai Et al.
    Bioact Mater  2022;18:138-150
  63. Transcription factor protein interactomes reveal genetic determinants in heart disease.
    Authors: Christine E Et al.
    Cell  2022;185:794-814.e30
  64. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids.
    Authors: Takashi Et al.
    Stem Cell Reports  2022;17:820-834
  65. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.
    Authors: Lu Et al.
    Nat Protoc  2022;17:2699-2719
  66. Spinal dI4 Interneuron Differentiation From Human Pluripotent Stem Cells.
    Authors: Hong Et al.
    Front Mol Neurosci  2022;15:845875
  67. Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses.
    Authors: Florent Et al.
    Viruses  2022;15
  68. The GSK-3 Inhibitor CT99021 Enhances the Acquisition of Spatial Learning and the Accuracy of Spatial Memory.
    Authors: Bong-Kiun Et al.
    Front Mol Neurosci  2022;14:804130
  69. DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagy.
    Authors: Catherine Et al.
    Brain  2022;145:4368-4384
  70. Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells.
    Authors: Francisco J Et al.
    Nat Commun  2022;13:7470
  71. Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features.
    Authors: Muhammad Abid Et al.
    Cell Death Dis  2022;13:670
  72. A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production.
    Authors: Zhen Et al.
    Nat Commun  2022;13:5491
  73. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.
    Authors: Beate Et al.
    Nat Commun  2022;13:4819
  74. A multimodal iPSC platform for cystic fibrosis drug testing.
    Authors: Mary Lou Et al.
    Nat Commun  2022;13:4270
  75. Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development.
    Authors: Vincent Et al.
    Nat Commun  2022;13:3861
  76. A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine.
    Authors: Miguel N Et al.
    Nat Commun  2022;13:1503
  77. Re-Expression of ERα and AR in Receptor Negative Endocrine Cancers via GSK3 Inhibition.
    Authors: Monica M Et al.
    Front Oncol  2022;12:824594
  78. Renalase and its receptor, PMCA4b, are expressed in the placenta throughout the human gestation.
    Authors: Christine Et al.
    Sci Rep  2022;12:4953
  79. Tumor endothelial marker 1 is upregulated in heart after cardiac injury and participates in cardiac remodeling.
    Authors: Yen-Wen Et al.
    Sci Rep  2022;12:10532
  80. Streamlined and quantitative detection of chimerism using digital PCR.
    Authors: Hiromitsu Et al.
    Sci Rep  2022;12:10223
  81. Isolation and characterization of human embryonic stem cell-derived heart field-specific cardiomyocytes unravels new insights into their transcriptional and electrophysiological profiles.
    Authors: Peng Et al.
    Cardiovasc Res  2022;118:828-843
  82. Genome-wide identification of the genetic basis of amyotrophic lateral sclerosis.
    Authors: Cheng Et al.
    Neuron  2022;110:992-1008.e11
  83. The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cells.
    Authors: Wendy Et al.
    Elife  2022;11
  84. EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation.
    Authors: Xi Et al.
    Elife  2022;11
  85. Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface.
    Authors: Sallie Et al.
    Elife  2022;11
  86. An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates.
    Authors: Alexandre P Et al.
    Elife  2022;11
  87. A Novel Bioreactor for Reconstitution of the Epithelium and Submucosal Glands in Decellularized Ferret Tracheas.
    Authors: Kalpaj R Et al.
    Cells  2022;11
  88. Canonical Wnt signaling and the regulation of divergent mesenchymal Fgf8 expression in axolotl limb development and regeneration.
    Authors: Elly Et al.
    Elife  2022;11
  89. Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells.
    Authors: Timothy C Et al.
    Elife  2022;11
  90. Tension-driven multi-scale self-organisation in human iPSC-derived muscle fibers.
    Authors: Erica Et al.
    Elife  2022;11
  91. Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signaling.
    Authors: Ran Et al.
    Elife  2022;11
  92. Kidney organoids recapitulate human basement membrane assembly in health and disease.
    Authors: Roy Et al.
    Elife  2022;11
  93. An In Vitro Microfluidic Alveolus Model to Study Lung Biomechanics.
    Authors: Shyni Et al.
    Front Bioeng Biotechnol  2022;10:848699
  94. Novel GSK-3 kinase inhibitor Pym-5 induces GSK-3β rather than GSK-3α-dependent melanogenesis in murine melanoma cells.
    Authors: Yin Et al.
    J Dermatol Sci  2022;106:170-180
  95. High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells.
    Authors: Di Et al.
    Front Cell Dev Biol  2021;9:656867
  96. SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.
    Authors: Andrew Et al.
    J Virol  2021;95:e0086221
  97. Expression of HIV-1 Intron-Containing RNA in Microglia Induces Inflammatory Responses.
    Authors: Gustavo Et al.
    J Virol  2021;95
  98. CHAF1A Blocks Neuronal Differentiation and Promotes Neuroblastoma Oncogenesis via Metabolic Reprogramming.
    Authors: Giovanni Et al.
    Adv Sci (Weinh)  2021;8:e2005047
  99. Elevated Extracellular cGMP Produced after Exposure to Enterotoxigenic Escherichia coli Heat-Stable Toxin Induces Epithelial IL-33 Release and Alters Intestinal Immunity.
    Authors: John D Et al.
    Infect Immun  2021;89
  100. NRF1 association with AUTS2-Polycomb mediates specific gene activation in the brain.
    Authors: Danny Et al.
    Mol Cell  2021;81:4663-4676.e8
  101. Evolutionary epigenomic analyses in mammalian early embryos reveal species-specific innovations and conserved principles of imprinting.
    Authors: Ling Et al.
    Sci Adv  2021;7:eabi6178
  102. Generation of human blastocyst-like structures from pluripotent stem cells.
    Authors: Wei Et al.
    Cell Discov  2021;7:81
  103. A diamidobenzimidazole STING agonist protects against SARS-CoV-2 infection.
    Authors: Fiachra H. Et al.
    Sci Immunol  2021;6
  104. NOTUM from Apc-mutant cells biases clonal competition to initiate cancer.
    Authors: Ari Et al.
    Nature  2021;594:430-435
  105. Neuronal enhancers are hotspots for DNA single-strand break repair.
    Authors: Rafael Et al.
    Nature  2021;593:440-444
  106. The connectome of neural crest enhancers reveals regulatory features of signaling systems.
    Authors: Marcos Et al.
    Dev Cell  2021;56:1268-1282.e6
  107. Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes.
    Authors: Bin Et al.
    Biochem Biophys Res Commun  2021;552:84-90
  108. Resolving cell state in iPSC-derived human neural samples with multiplexed fluorescence imaging.
    Authors: Beth A Et al.
    Commun Biol  2021;4:786
  109. TENET: gene network reconstruction using transfer entropy reveals key regulatory factors from single cell transcriptomic data.
    Authors: Kyoung-Jae Et al.
    Nucleic Acids Res  2021;49:e1
  110. A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.
    Authors: John M Et al.
    EMBO J  2021;40:e103811
  111. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program.
    Authors: Takuya Et al.
    Life Sci Alliance  2021;4
  112. Paediatric gastric organoids as a tool for disease modelling and clinical translation.
    Authors: Paolo Et al.
    Pediatr Surg Int  2021;37:317-324
  113. Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis.
    Authors: Beth Y Et al.
    Cell Rep  2021;37:110146
  114. Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage.
    Authors: Kolja Et al.
    Cell Rep  2021;37:110068
  115. HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
    Authors: Digvijay Et al.
    Science  2021;371
  116. Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease.
    Authors: Andrew Et al.
    Cell Rep  2021;36:109636
  117. Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2.
    Authors: J Travis Et al.
    Cell Rep  2021;36:109512
  118. A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy.
    Authors: Anne G Et al.
    Cell Rep  2021;36:109360
  119. Active elimination of intestinal cells drives oncogenic growth in organoids.
    Authors: Jacco Et al.
    Cell Rep  2021;36:109307
  120. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment.
    Authors: Paul Et al.
    Cell Rep  2021;35:109088
  121. 5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer's disease.
    Authors: Li Et al.
    Cell Rep  2021;35:109042
  122. Airway basal stem cells generate distinct subpopulations of PNECs.
    Authors: Ying Et al.
    Cell Rep  2021;35:109011
  123. Generation of mixed murine organoids to model cellular interactions.
    Authors: Jacco Et al.
    STAR Protoc  2021;2:100997
  124. Differentiation of human pluripotent stem cells into functional airway basal stem cells.
    Authors: Shingo Et al.
    STAR Protoc  2021;2:100683
  125. High-throughput generation of midbrain dopaminergic neuron organoids from reporter human pluripotent stem cells.
    Authors: Tim Et al.
    STAR Protoc  2021;2:100463
  126. Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells.
    Authors: Andras Et al.
    Cell Stem Cell  2021;28:79-95.e8
  127. Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use.
    Authors: Kim Et al.
    Cell Stem Cell  2021;28:343
  128. Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.
    Authors: Jamie A Et al.
    Cell Stem Cell  2021;28:209-216.e4
  129. Generation of Functional Organs Using a Cell-Competitive Niche in Intra- and Inter-species Rodent Chimeras.
    Authors: Hiromitsu Et al.
    Cell Stem Cell  2021;28:141-149.e3
  130. Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function
    Authors: Xiong Et al.
    cell stem Cell  2021;28:112
  131. Bioengineered optogenetic model of human neuromuscular junction.
    Authors: Bruce R Et al.
    Biomaterials  2021;276:121033
  132. Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing.
    Authors: Deborah A Et al.
    SLAS Technol  2021;26:287-299
  133. Pharmacological targeting of Sam68 functions in colorectal cancer stem cells.
    Authors: Yannick D Et al.
    iScience  2021;24:103442
  134. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury.
    Authors: Philip J Et al.
    iScience  2021;24:102827
  135. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.
    Authors: Ruili Et al.
    Methods Mol Biol  2021;2454:811-827
  136. Evaluating totipotency using criteria of increasing stringency.
    Authors: Andrea Et al.
    Nat Cell Biol  2021;23:49-60
  137. Bioactive Lipid O-cyclic phytosphingosine-1-phosphate Promotes Differentiation of Human Embryonic Stem Cells into Cardiomyocytes via ALK3/BMPR Signaling.
    Authors: Su-Jin Et al.
    Int J Mol Sci  2021;22
  138. In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.
    Authors: Anestis Et al.
    Curr Protoc  2021;1:e244
  139. Differentiation of Brain Pericyte-Like Cells from Human Pluripotent Stem Cell-Derived Neural Crest.
    Authors: Sean P Et al.
    Curr Protoc  2021;1:e21
  140. Generating Enteric Nervous System Progenitors from Human Pluripotent Stem Cells.
    Authors: Anestis Et al.
    Curr Protoc  2021;1:e137
  141. Direct SARS-CoV-2 infection of the human inner ear may underlie COVID-19-associated audiovestibular dysfunction.
    Authors: Karen E Et al.
    Commun Med (Lond)  2021;1:44
  142. Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes.
    Authors: Jason G Et al.
    Nat Methods  2021;18:507-519
  143. An early cell shape transition drives evolutionary expansion of the human forebrain.
    Authors: Ulrich Et al.
    Cell  2021;184:2084-2102.e19
  144. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.
    Authors: Juan Carlos Et al.
    Cell  2021;184:2020-2032.e14
  145. The histone demethylase KDM2B regulates human primordial germ cell-like cells specification.
    Authors: Yi Et al.
    Int J Biol Sci  2021;17:527-538
  146. Human Pluripotent Stem Cell-Derived Intestinal Organoids Model SARS-CoV-2 Infection Revealing a Common Epithelial Inflammatory Response.
    Authors: Gustavo Et al.
    Stem Cell Reports  2021;16:940-953
  147. Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development.
    Authors: Madeline A Et al.
    Nat Protoc  2021;16:579-602
  148. Generation of mouse-human chimeric embryos.
    Authors: Boyang Et al.
    Nat Protoc  2021;16:3954-3980
  149. The miR-199a/214 Cluster Controls Nephrogenesis and Vascularization in a Human Embryonic Stem Cell Model.
    Authors: Adrian S Et al.
    Stem Cell Reports  2021;16:134-148
  150. Mechanical stimulation enhances development of scaffold-free, 3D-printed, engineered heart tissue grafts.
    Authors: Yun Et al.
    J Tissue Eng Regen Med  2021;15:503-512
  151. The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.
    Authors: Ha Neui Et al.
    Neurobiol Dis  2021;152:105294
  152. Pharmacological Manipulation of Wnt/β-Catenin Signaling Pathway in Human Neural Precursor Cells Alters Their Differentiation Potential and Neuronal Yield.
    Authors: Michael Et al.
    Front Mol Neurosci  2021;14:680018
  153. Characterization of primary models of human trophoblast.
    Authors: Qian Et al.
    Development  2021;148
  154. Induced pluripotent stem cell-derived motor neurons of CMT type 2 patients reveal progressive mitochondrial dysfunction.
    Authors: Ludo Et al.
    Brain  2021;144:2471-2485
  155. Mitochondrial bioenergetic deficits in C9orf72 amyotrophic lateral sclerosis motor neurons cause dysfunctional axonal homeostasis.
    Authors: David Et al.
    Acta Neuropathol  2021;141:257-279
  156. Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identity in vitro.
    Authors: Arun Et al.
    Cell Syst  2021;12:885-899.e8
  157. Host metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infection.
    Authors: Xinwen Et al.
    Protein Cell  2021;12:717-733
  158. Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors.
    Authors: Konstantinos-Dionysios Et al.
    Nat Commun  2021;12:6636
  159. Generation of functional ciliated cholangiocytes from human pluripotent stem cells.
    Authors: Marcela Et al.
    Nat Commun  2021;12:6504
  160. Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis.
    Authors: Alexander Et al.
    Bio Protoc  2021;11:e4042
  161. Prednisolone rescues Duchenne muscular dystrophy phenotypes in human pluripotent stem cell-derived skeletal muscle in vitro.
    Authors: Bianca Et al.
    Proc Natl Acad Sci U S A  2021;118
  162. In vitro teratogenicity testing using a 3D, embryo-like gastruloid system.
    Authors: Alfonso Martinez Et al.
    Reprod Toxicol  2021;105:72-90
  163. Phenotypic plasticity underlies local invasion and distant metastasis in colon cancer.
    Authors: Won Kyu Et al.
    Elife  2021;10
  164. Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients.
    Authors: Ida Et al.
    Elife  2021;10
  165. Wnt signaling mediates acquisition of blood-brain barrier properties in naïve endothelium derived from human pluripotent stem cells.
    Authors: Britta Et al.
    Elife  2021;10
  166. NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes.
    Authors: Anne G Et al.
    Elife  2021;10
  167. A high-content platform for physiological profiling and unbiased classification of individual neurons.
    Authors: Brian J Et al.
    Cell Rep Methods  2021;1
  168. 16p11.2 microdeletion imparts transcriptional alterations in human iPSC-derived models of early neural development.
    Authors: Hui Et al.
    Elife  2020;9
  169. The combination of forskolin and VPA increases gene expression efficiency to the hypoxia/neuron-specific system.
    Authors: Janghwan Et al.
    Ann Transl Med  2020;8:933
  170. Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis.
    Authors: Joshy Et al.
    Mol Cell  2020;80:648-665.e9
  171. SEM/FIB Imaging for Studying Neural Interfaces.
    Authors: Amos Et al.
    Dev Neurobiol  2020;80:305-315
  172. Electrophysiologic Characterization of Developing Human Embryonic Stem Cell-Derived Photoreceptor Precursors.
    Authors: Alon Et al.
    Invest Ophthalmol Vis Sci  2020;61:44
  173. A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract.
    Authors: Joshua M Et al.
    Sci Adv  2020;6
  174. Human Bocavirus 1 Infection of Well-Differentiated Human Airway Epithelium.
    Authors: Jianming Et al.
    Curr Protoc Microbiol  2020;58:e107
  175. Inhibition of LTβR signalling activates WNT-induced regeneration in lung.
    Authors: Oliver Et al.
    Nature  2020;588:151-156
  176. Intracellular pH controls WNT downstream of glycolysis in amniote embryos.
    Authors: Masayuki Et al.
    Nature  2020;584:98-101
  177. An in vitro model of early anteroposterior organization during human development.
    Authors: Tina Et al.
    Nature  2020;582:410-415
  178. In vitro characterization of the human segmentation clock.
    Authors: Christoph Et al.
    Nature  2020;580:113-118
  179. Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells.
    Authors: Yu-Hwai Et al.
    Dev Cell  2020;54:516-528.e7
  180. Persistent DNA Damage and Senescence in the Placenta Impacts Developmental Outcomes of Embryos.
    Authors: Vijay Pratap Et al.
    Dev Cell  2020;54:333-347.e7
  181. Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development.
    Authors: Carissa Et al.
    Dev Cell  2020;53:229-239.e7
  182. Intravital three-dimensional bioprinting.
    Authors: Valentina Et al.
    Nat Biomed Eng  2020;4:901-915
  183. CRISPR-mediated gene correction links the ATP7A M1311V mutations with amyotrophic lateral sclerosis pathogenesis in one individual.
    Authors: Robert Et al.
    Commun Biol  2020;3:33
  184. Long-term expansion with germline potential of human primordial germ cell-like cells in vitro.
    Authors: Takuya Et al.
    EMBO J  2020;39:e104929
  185. Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells.
    Authors: Da-Zhi Et al.
    EMBO J  2020;39:e104324
  186. Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population.
    Authors: Walter C Et al.
    Stem Cells  2020;38:1400-1408
  187. Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms.
    Authors: Alexander R Et al.
    J Vac Sci Technol A  2020;38:033205
  188. Regulation of Tumor Initiation by the Mitochondrial Pyruvate Carrier.
    Authors: Claudio J Et al.
    Cell Metab  2020;31:284-300.e7
  189. Differentiation of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell-Derived Ureteric Bud and Ex Fetu Mesenchyme.
    Authors: Lorna Et al.
    J Am Soc Nephrol  2020;31:2253-2262
  190. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells.
    Authors: Constanze Et al.
    Cell Rep  2020;31:107691
  191. Evaluating the Effects of Cell Sorting on Gene Expression.
    Authors: Peter Et al.
    J Biomol Tech  2020;31:100-111
  192. High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis.
    Authors: Preethi Et al.
    Cell Rep  2020;30:2055-2064.e5
  193. SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response.
    Authors: Mary Lou Et al.
    Cell Stem Cell  2020;27:962-973.e7
  194. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids.
    Authors: Donna L Et al.
    Cell Stem Cell  2020;27:951-961.e5
  195. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2.
    Authors: Byeong-Sun Et al.
    Cell Stem Cell  2020;27:905-919.e10
  196. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction.
    Authors: Richard C Et al.
    Cell Stem Cell  2020;27:890-904.e8
  197. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development.
    Authors: Richard A Et al.
    Cell Stem Cell  2020;27:822-839.e8
  198. Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity.
    Authors: Mark Et al.
    Cell Stem Cell  2020;27:813-821.e6
  199. Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells.
    Authors: Andrew Et al.
    Cell Stem Cell  2020;27:663-678.e8
  200. Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation.
    Authors: Ludovic Et al.
    Cell Stem Cell  2020;27:470-481.e6
  201. Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency.
    Authors: Steven C Et al.
    Cell Stem Cell  2020;27:459-469.e8
  202. A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.
    Authors: Katja Et al.
    Cell Stem Cell  2020;27:158-176.e10
  203. A Non-Coding Disease Modifier of Pancreatic Agenesis Identified by Genetic Correction in a Patient-Derived iPSC Line.
    Authors: Paul Et al.
    Cell Stem Cell  2020;27:137-146.e6
  204. Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson's Disease.
    Authors: Minhyung Et al.
    Molecules  2020;25
  205. Overcoming Wnt-β-catenin dependent anticancer therapy resistance in leukaemia stem cells.
    Authors: Sheng Et al.
    Nat Cell Biol  2020;22:689-700
  206. Genome Editing-Mediated Utrophin Upregulation in Duchenne Muscular Dystrophy Stem Cells.
    Authors: Melissa J Et al.
    Mol Ther Nucleic Acids  2020;22:500-509
  207. Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos.
    Authors: Krisztina Et al.
    Methods Mol Biol  2020;2258:131-147
  208. Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting.
    Authors: Narutoshi Et al.
    Methods Mol Biol  2020;2140:183-197
  209. Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke.
    Authors: Jeong-Eun Et al.
    Stem Cells Int  2020;2020:4061516
  210. Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia.
    Authors: Stewart A Et al.
    STAR Protoc  2020;1:100190
  211. Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.
    Authors: April D Et al.
    STAR Protoc  2020;1:100158
  212. Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.
    Authors: Constanze Et al.
    STAR Protoc  2020;1:100130
  213. Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
    Authors: Koen C Et al.
    STAR Protoc  2020;1:100126
  214. FOXO3 targets are reprogrammed as Huntington's disease neural cells and striatal neurons face senescence with p16INK4a increase.
    Authors: Lisa M Et al.
    Aging Cell  2020;19:e13226
  215. Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation.
    Authors: Micha Et al.
    BMC Biol  2020;18:42
  216. Bioelectrical domain walls in homogeneous tissues.
    Authors: Olivier Et al.
    Nat Phys  2020;16:357-364
  217. Differentiation of PTH-Expressing Cells From Human Pluripotent Stem Cells.
    Authors: Michael A Et al.
    Endocrinology  2020;161
  218. Establishment and differentiation of long-term trophoblast organoid cultures from the human placenta.
    Authors: Lucy Et al.
    Nat Protoc  2020;15:3441-3463
  219. Generation of human oogonia from induced pluripotent stem cells in culture.
    Authors: Kotaro Et al.
    Nat Protoc  2020;15:1560-1583
  220. Hedgehog Activation Regulates Human Osteoblastogenesis.
    Authors: Takashi Et al.
    Stem Cell Reports  2020;15:125-139
  221. Pluripotent stem cell derived intestinal organoids with an enteric nervous system.
    Authors: Maxime M Et al.
    Methods Cell Biol  2020;159:175-199
  222. Human Motor Neurons With SOD1-G93A Mutation Generated From CRISPR/Cas9 Gene-Edited iPSCs Develop Pathological Features of Amyotrophic Lateral Sclerosis.
    Authors: Lee J Et al.
    Front Cell Neurosci  2020;14:604171
  223. Neural Transplants From Human Induced Pluripotent Stem Cells Rescue the Pathology and Behavioral Defects in a Rodent Model of Huntington's Disease.
    Authors: Hyun Jung Et al.
    Front Neurosci  2020;14:558204
  224. Nucleosides Rescue Replication-Mediated Genome Instability of Human Pluripotent Stem Cells.
    Authors: Peter W Et al.
    Stem Cell Reports  2020;14:1009-1017
  225. Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro.
    Authors: Emanuela Et al.
    Development  2020;147
  226. Aberrant Differentiation of Human Pluripotent Stem Cell-Derived Kidney Precursor Cells inside Mouse Vascularized Bioreactors.
    Authors: Adrian S Et al.
    Nephron  2020;144:509-524
  227. Robust and Reproducible Generation of Induced Neural Stem Cells from Human Somatic Cells by Defined Factors.
    Authors: Chan Young Et al.
    Int J Stem Cells  2020;13:80-92
  228. Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells.
    Authors: Nicholas C Et al.
    Curr Protoc Immunol  2020;131:e113
  229. Disrupted ER membrane protein complex-mediated topogenesis drives congenital neural crest defects.
    Authors: Mustafa K Et al.
    J Clin Invest  2020;130:813-826
  230. A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG.
    Authors: Qianqian Et al.
    Stem Cell Res Ther  2020;11:67
  231. Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis.
    Authors: Frédéric H-T Et al.
    Nat Commun  2020;11:6341
  232. Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells.
    Authors: Yuka Et al.
    Nat Commun  2020;11:5656
  233. Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause.
    Authors: Rui Et al.
    Nat Commun  2020;11:5499
  234. Generation and trapping of a mesoderm biased state of human pluripotency.
    Authors: Jason Et al.
    Nat Commun  2020;11:4989
  235. Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1.
    Authors: Malcolm G Et al.
    Cell Mol Gastroenterol Hepatol  2020;11:465-489
  236. Elevated de novo protein synthesis in FMRP-deficient human neurons and its correction by metformin treatment.
    Authors: Maija L Et al.
    Mol Autism  2020;11:41
  237. BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.
    Authors: Xavier Et al.
    Cell Death Dis  2020;11:378
  238. Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.
    Authors: Darrell N Et al.
    Nat Commun  2020;11:215
  239. Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells.
    Authors: Igor I Et al.
    Bio Protoc  2020;10:e3675
  240. Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives.
    Authors: Sun Et al.
    Sci Rep  2020;10:3939
  241. Autologous correction in patient induced pluripotent stem cell-endothelial cells to identify a novel pathogenic mutation of hereditary hemorrhagic telangiectasia.
    Authors: Yanyan Et al.
    Pulm Circ  2020;10:2045894019885357
  242. Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming.
    Authors: Thaddeus G Et al.
    Sci Rep  2020;10:19159
  243. A method for differentiating human induced pluripotent stem cells toward functional cardiomyocytes in 96-well microplates.
    Authors: Gareth Et al.
    Sci Rep  2020;10:18498
  244. Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy.
    Authors: Edwin M Et al.
    Am J Hum Genet  2020;107:278-292
  245. Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions.
    Authors: Jizhong Et al.
    STAR Protoc  2020;1
  246. Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9.
    Authors: Ting Et al.
    STAR Protoc  2020;1
  247. Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro.
    Authors: Emanuela Et al.
    Development  2020;
  248. Evaluating the Effects of Cell Sorting on Gene Expression.
    Authors: Peter Et al.
    J Biomol Tech  2020;
  249. Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.
    Authors: Frith and Tsakiridis
    Curr Protoc Stem Cell Biol  2019;:e81
  250. Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes.
    Authors: Eva Et al.
    Bio Protoc  2019;9:e3312
  251. User-defined morphogen patterning for directing human cell fate stratification.
    Authors: Regier Et al.
    Sci Rep  2019;9:6433
  252. Enteroendocrine Progenitor Cell-Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner.
    Authors: Ajeet P Et al.
    Cell Mol Gastroenterol Hepatol  2019;9:447-464
  253. The role of cardiac transcription factor NKX2-5 in regulating the human cardiac miRNAome.
    Authors: David J Et al.
    Sci Rep  2019;9:15928
  254. Quantification of human neuromuscular function through optogenetics.
    Authors: Vila Et al.
    Theranostics  2019;9:1232
  255. Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions.
    Authors: Regent Et al.
    Sci Rep  2019;9:10646
  256. Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium.
    Authors: Suzuki Et al.
    Sci Rep  2019;9:10506
  257. Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model.
    Authors: Ranganathan Et al.
    Infect Immun  2019;87
  258. Human blood vessel organoids as a model of diabetic vasculopathy.
    Authors: Wimmer Et al.
    Nature  2019;565:505
  259. Canonical Wnt5b Signaling Directs Outlying Nkx2.5+ Mesoderm into Pacemaker Cardiomyocytes.
    Authors: Peidong Et al.
    Dev Cell  2019;50:729-743.e5
  260. GSK3 regulates hair cell fate in the developing mammalian cochlea.
    Authors: Matthew W Et al.
    Dev Biol  2019;453:191-205
  261. Human neural crest induction by temporal modulation of WNT activation.
    Authors: Martín I Et al.
    Dev Biol  2019;449:99-106
  262. Specification of positional identity in forebrain organoids.
    Authors: Ryan M Et al.
    Nat Biotechnol  2019;37:436-444
  263. Cardiomyocyte functional screening: interrogating comparative electrophysiology of high-throughput model cell systems.
    Authors: James R Et al.
    Am J Physiol Cell Physiol  2019;317:C1256-C1267
  264. Wnt signaling inhibition by monensin results in a period of Hippo pathway activation during intestinal adaptation in zebrafish.
    Authors: Tracy C Et al.
    Am J Physiol Gastrointest Liver Physiol  2019;316:G679-G691
  265. A C/EBPα-Wnt connection in gut homeostasis and carcinogenesis.
    Authors: Heuberger Et al.
    Life Sci Alliance  2019;2:e201800173
  266. NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content.
    Authors: Chiu Et al.
    Cell Rep  2019;28:332
  267. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
    Authors: Genga Et al.
    Cell Rep  2019;27:708
  268. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors.
    Authors: Yap Et al.
    Cell Rep  2019;26:3231
  269. Development of MAP4 Kinase Inhibitors as Motor Neuron-Protecting Agents.
    Authors: Sebastian Et al.
    Cell Chem Biol  2019;26:1703-1715.e37
  270. User-Friendly and Parallelized Generation of Human Induced Pluripotent Stem Cell-Derived Microtissues in a Centrifugal Heart-on-a-Chip.
    Authors: Schneider Et al.
    Tissue Eng Part A  2019;25:786
  271. Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs.
    Authors: Guangming Et al.
    Cell Stem Cell  2019;25:737-753.e4
  272. Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis.
    Authors: Marc Et al.
    Nat Med  2019;25:1873-1884
  273. Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity.
    Authors: Thomas C Et al.
    Cell Stem Cell  2019;25:103-119.e6
  274. Single-Cell Sequencing of iPSC-DA Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
    Authors: Lang Et al.
    Cell Stem Cell  2019;24:93
  275. Establishment of porcine and human expanded potential stem cells.
    Authors: Gao Et al.
    Nat Cell Biol  2019;21:687
  276. Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells.
    Authors: Fan Et al.
    Int J Mol Sci  2019;21
  277. End-to-End Platform for Human Pluripotent Stem Cell Manufacturing.
    Authors: Puspa R Et al.
    Int J Mol Sci  2019;21
  278. Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs. Small Molecule-Based Approaches.
    Authors: Varghese Et al.
    Stem Cells Int  2019;2019:5968236
  279. A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.
    Authors: Canizo Et al.
    BMC Dev Biol  2019;19:13
  280. An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons.
    Authors: Canfield Et al.
    Fluids Barriers CNS  2019;16:25
  281. Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.
    Authors: Haidar Et al.
    Autophagy  2019;15:1051
  282. Activation of Hedgehog Signaling Promotes Development of Mouse and Human Enteric Neural Crest Cells, Based on Single-Cell Transcriptome Analyses.
    Authors: Chi-chung Et al.
    Gastroenterology  2019;157:1556-1571.e5
  283. In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells.
    Authors: Pentao Et al.
    Nat Protoc  2019;14:350-378
  284. Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells.
    Authors: Konstantinos-Dionysios Et al.
    Nat Protoc  2019;14:3303-3332
  285. Generation of blood vessel organoids from human pluripotent stem cells.
    Authors: Josef M Et al.
    Nat Protoc  2019;14:3082-3100
  286. Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype.
    Authors: Kumi Et al.
    Nat Protoc  2019;14:2781-2817
  287. Derivation of enteric neuron lineages from human pluripotent stem cells.
    Authors: Kevin Et al.
    Nat Protoc  2019;14:1261-1279
  288. Huntington's Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support.
    Authors: Garcia Et al.
    Front Neurosci  2019;13:669
  289. Cardiac regeneration using human-induced pluripotent stem cell-derived biomaterial-free 3D-bioprinted cardiac patch in vivo.
    Authors: Steven M Et al.
    J Tissue Eng Regen Med  2019;13:2031-2039
  290. Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm.
    Authors: Kyoung Hwa Et al.
    J Cell Sci  2019;132
  291. FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
    Authors: Hackland Et al.
    Stem Cell Reports  2019;12:920
  292. Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways.
    Authors: Yin Et al.
    Stem Cell Reports  2019;12:488
  293. Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.
    Authors: Zhang Et al.
    Nat Commun  2019;10:496
  294. Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals.
    Authors: Gentsch Et al.
    Nat Commun  2019;10:4269
  295. Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction.
    Authors: Park Et al.
    Nat Commun  2019;10:3123
  296. Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg-/- mice.
    Authors: Du Et al.
    Protein Cell  2019;10:31
  297. Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system.
    Authors: Xu Et al.
    Stem Cell Res Ther  2019;10:193
  298. Cortical Neurogenesis Requires Bcl6-Mediated Transcriptional Repression of Multiple Self-Renewal-Promoting Extrinsic Pathways.
    Authors: Francois Et al.
    Neuron  2019;103:1096-1108.e4
  299. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium.
    Authors: Olga Et al.
    J Vis Exp  2019;
  300. Differentiation and Contractile Analysis of GFP-Sarcomere Reporter hiPSC-Cardiomyocytes.
    Authors: Christine E Et al.
    Curr Protoc Hum Genet  2018;96:21.12.1-21.12.12
  301. ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes.
    Authors: Shekhar Et al.
    Sci Rep  2018;8:9944
  302. Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids.
    Authors: Srikanth Et al.
    Transl Psychiatry  2018;8:77
  303. Stage-specific Effects of Bioactive Lipids on Human iPSC Cardiac Differentiation and Cardiomyocyte Proliferation.
    Authors: Sharma Et al.
    Sci Rep  2018;8:6618
  304. Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
    Authors: Levardon Et al.
    Bio Protoc  2018;8
  305. Functional comparison of distinct Brachyury+ states in a renal differentiation assay.
    Authors: Zhou Et al.
    Biol Open  2018;7
  306. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers.
    Authors: Shafa Et al.
    Front Med (Lausanne)  2018;5:69
  307. Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.
    Authors: McCauley Et al.
    Curr Protoc Stem Cell Biol  2018;45:e51
  308. Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.
    Authors: Jonathan G Et al.
    Dev Cell  2018;44:87-96.e5
  309. APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway.
    Authors: Bin Et al.
    Dev Cell  2018;44:566-581.e8
  310. Generation of human vascularized brain organoids.
    Authors: Pham Et al.
    Neuroreport  2018;29:588
  311. Identification of direct negative cross-talk between the SLIT2 and bone morphogenetic protein-Gremlin signaling pathways.
    Authors: Tumelty Et al.
    J Biol Chem  2018;293:3039
  312. 3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds.
    Authors: James R Et al.
    Adv Funct Mater  2018;28
  313. Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.
    Authors: Schmitt Et al.
    Cell Rep  2018;24:2312
  314. Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics.
    Authors: Haojia Et al.
    Cell Stem Cell  2018;23:869-881.e8
  315. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.
    Authors: Ying Et al.
    Cell Stem Cell  2018;23:516-529.e5
  316. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
    Authors: Guangming Et al.
    Cell Stem Cell  2018;23:266-275.e6
  317. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell  2018;23:123
  318. Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.
    Authors: Rajamani Et al.
    Cell Stem Cell  2018;22:698
  319. Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.
    Authors: Tata Et al.
    Cell Stem Cell  2018;22:668
  320. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
    Authors: Lynch Et al.
    Cell Stem Cell  2018;22:653
  321. CRISPR-based chromatin remodeling of the endogenous Oct4 or Sox2 locus enables reprogramming to pluripotency.
    Authors: Liu Et al.
    Cell Stem Cell.  2018;22:252
  322. iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.
    Authors: Wei Et al.
    Cell  2018;173:851-863.e16
  323. Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles.
    Authors: Yamoah Et al.
    Int J Nanomedicine  2018;13:6073
  324. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle.
    Authors: George A Et al.
    J Tissue Eng Regen Med  2018;12:e918-e936
  325. The COPII cargo adapter SEC24C is essential for neuronal homeostasis.
    Authors: Wang Et al.
    J Clin Invest  2018;128:3319
  326. Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors.
    Authors: Bantounas Et al.
    Stem Cell Reports  2018;10:766
  327. Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors.
    Authors: Bar-Nur Et al.
    Stem Cell Reports  2018;10:1505
  328. Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation.
    Authors: Li Et al.
    Protein Cell  2018;
  329. Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.
    Authors: Leung Et al.
    Stem Cells  2018;
  330. Cocktail of chemical compounds robustly promoting cell reprogramming protects liver against acute injury.
    Authors: Tang and Cheng
    Protein Cell  2017;8:273
  331. Culture in Glucose-Depleted Medium Supplemented with Fatty Acid and 3,3',5-Triiodo-l-Thyronine Facilitates Purification and Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes.
    Authors: Lin Et al.
    Front Endocrinol (Lausanne)  2017;8:253
  332. Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinalstem cell niche.
    Authors: Yang
    Nat Commun  2017;8:14766
  333. A pluripotent stem cell-based model for post-implantation human amniotic sac development.
    Authors: Shao
    Nat Commun  2017;8(1):208
  334. Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes.
    Authors: Ong Et al.
    Sci Rep  2017;7:4566
  335. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage.
    Authors: Christoforou Et al.
    Sci Rep  2017;7:40285
  336. Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity.
    Authors: Liam R Et al.
    Curr Protoc Toxicol  2017;73:22.5.1-22.5.22
  337. Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
    Authors: Wen Et al.
    Stem Cells Transl Med  2017;6:527-538
  338. Establishment of mouse expanded potential stem cells.
    Authors: Yang Et al.
    Nature  2017;550:393
  339. Guided self-organization and cortical plate formation in human brain organoids.
    Authors: Lancaster Et al.
    Nat Biotechnol  2017;35:659
  340. Efficient differentiation of TBX18(+)/WT1(+) epicardial-like cells from human pluripotent stem cells using small molecular compounds.
    Authors: Zhao Et al.
    Stem Cells Dev  2017;26:528
  341. Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells.
    Authors: Walsh Et al.
    Cell Transplant  2017;26:1890
  342. Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.
    Authors: Vermilyea Et al.
    Stem Cells Dev  2017;26:1225
  343. Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9.
    Authors: Kirmo Et al.
    Stem Cell Res  2017;22:16-19
  344. Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
    Authors: Kotton Et al.
    Cell Stem Cell.  2017;21:472
  345. Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming.
    Authors: Atsushi Et al.
    Cell Stem Cell  2017;21:456-471.e5
  346. A modular platform for differentiation of human PSCs into all major ectodermal lineages.
    Authors: Tchieu Et al.
    Cell Stem Cell.  2017;21:399
  347. Live imaging reveals that the first division of differentiating human embryonic stem cells often yields asymmetric fates.
    Authors: Brown
    Cell Rep  2017;21(2):301
  348. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling.
    Authors: McCauley Et al.
    Cell Stem Cell  2017;20:844
  349. In Vivo Human Somitogenesis Guides Somite Development from hPSCs.
    Authors: Xi
    Cell Rep.  2017;18(6):1573
  350. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.
    Authors: Bradley E Et al.
    Cell  2017;171:1611-1624.e24
  351. Anatomically and Functionally Distinct Lung Mesenchymal Populations Marked by Lgr5 and Lgr6.
    Authors: Lee Et al.
    Cell  2017;170:1149
  352. Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche.
    Authors: Kenichiro Et al.
    Nat Mater  2017;16:419-425
  353. Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency.
    Authors: Yang Et al.
    Cell.  2017;169:243
  354. Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural Crest Cell Function.
    Authors: Lai Et al.
    Gastroenterology  2017;153:139
  355. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells.
    Authors: Sampaziotis Et al.
    Nat Protoc  2017;12:814
  356. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
    Authors: Morizane and Bonventre
    Nat Protoc  2017;12:195
  357. Dynamic regulation of Nanog and stem cell-signaling pathways by Hoxa1 during early neuro-ectodermal differentiation of ES cells.
    Authors: Kumar Et al.
    Proc Natl Acad Sci U S A  2017;114:5838
  358. Modeling renal progenitors - defining the niche.
    Authors: Alan O Et al.
    Differentiation  2016;91:152-8
  359. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.
    Authors: Long Et al.
    Nat Biotechnol  2016;7:10354
  360. Generating trunk neural crest from human pluripotent stem cells.
    Authors: Huang Et al.
    PLoS One  2016;6:19727
  361. Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease.
    Authors: Fattahi Et al.
    Nature  2016;531:105
  362. Generation of serotonin neurons from human pluripotent stem cells.
    Authors: Lu Et al.
    Nat Biotechnol  2016;34:89
  363. Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells.
    Authors: Capowski Et al.
    Stem Cells  2016;34:2625
  364. Enhancing a Wnt-Telomere Feedback Loop Restores Intestinal Stem Cell Function in a Human Organotypic Model of Dyskeratosis Congenita
    Authors: Woo Et al.
    Cell Stem Cell  2016;19:397
  365. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell  2016;19:217
  366. Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation.
    Authors: Zhang Et al.
    Cell Stem Cell  2016;18:653
  367. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
    Authors: Foulke-Abel Et al.
    Nat Commun  2016;150:638
  368. Tankyrase inhibition promotes a stable human na�ve pluripotent state with improved functionality.
    Authors: Zimmerlin Et al.
    Development  2016;143:4368
  369. WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate.
    Authors: Leung Et al.
    Development  2016;143:398
  370. Allelic Imbalance in Regulation of ANRIL through Chromatin Interaction at 9p21 Endometriosis Risk Locus.
    Authors: Nakaoka Et al.
    PLoS Genet  2016;12:e1005893
  371. Efficient and Controlled Generation of 2D and 3D Bile Duct Tissue from Human Pluripotent Stem Cell-Derived Spheroids.
    Authors: Zhaohui Et al.
    Stem Cell Rev Rep  2016;12:500-8
  372. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Authors: Chal Et al.
    Nat.Protoc.  2016;11:1833
  373. Generation of kidney organoids from human pluripotent stem cells.
    Authors: Takasato Et al.
    Nature Protocols  2016;11:1681
  374. GH is permissive for neoplastic colon growth.
    Authors: Chesnokova Et al.
    Proc Natl Acad Sci U S A  2016;113:E3250
  375. HP Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
    Authors: Lin Et al.
    Stem Cells Transl Med  2016;
  376. Human pluripotent stem cell derived midbrain PITX3(eGFP/w) neurons: a versatile tool for pharmacological screening and neurodegenerative modeling.
    Authors: Watmuff Et al.
    Front Cell Neurosci  2015;9:104
  377. Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells.
    Authors: Mutze Et al.
    Cell Rep  2015;8:877
  378. Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.
    Authors: Jain Et al.
    Nat Commun  2015;6:6727
  379. Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells.
    Authors: Du Et al.
    Nat.Commun.  2015;6:6626
  380. Inhibition of Excessive Monoamine Oxidase A/B Activity Protects Against Stress-induced Neuronal Death in Huntington Disease.
    Authors: Ooi Et al.
    Nat Chem Biol  2015;52:1850
  381. Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines.
    Authors: Nostro Et al.
    Eur J Immunol  2015;4:591
  382. The lymph node as a new site for kidney organogenesis.
    Authors: Francipane and Lagasse
    Stem Cells Transl Med  2015;4:295
  383. Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter.
    Authors: Hill Et al.
    Mol Neurobiol  2015;45:1103
  384. Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
    Authors: Morizane Et al.
    Stem Cell Reports  2015;33:1193
  385. Phosphorylation and SCF-mediated degradation regulate CREB-H transcription of metabolic targets.
    Authors: Barbosa Et al.
    Stem Cell Reports  2015;26:2939
  386. Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System.
    Authors: Ying Et al.
    Stem Cells Dev  2015;24:2925-42
  387. Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.
    Authors: Huebsch Et al.
    Tissue Eng Part C Methods  2015;21:467
  388. Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons.
    Authors: Zhang Et al.
    Cell Stem Cell  2015;17:735
  389. Non-genomic and Immune Evolution of Melanoma Acquiring MAPKi Resistance.
    Authors: Hugo Et al.
    Cell  2015;162:1271
  390. GSK3β-Dzip1-Rab8 cascade regulates ciliogenesis after mitosis.
    Authors: Zhang Et al.
    PLoS Biol  2015;13:e1002129
  391. Functional annotation of native enhancers with a Cas9-histone demethylase fusion.
    Authors: Kearns Et al.
    Nat Methods  2015;12:401
  392. Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate.
    Authors: Srikanth Et al.
    Sci Rep  2015;12:1414
  393. The bimodally expressed microRNA miR-142 gates exit from pluripotency.
    Authors: Sladitschek and Neveu
    J Biol Chem  2015;11:850
  394. GSK-3 modulates cellular responses to a broad spectrum of kinase inhibitors.
    Authors: Thorne Et al.
    Mol Biol Cell  2015;11:58
  395. Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells' Transcription Factors.
    Authors: Solari Et al.
    Mol Syst Biol  2015;10:e0144336
  396. Reprogramming fibroblasts toward cardiomyocytes, neural stem cells and hepatocytes by cell activation and signaling-directed lineage conversion.
    Authors: Haixia Et al.
    Nat Protoc  2015;10:959-73
  397. Transcriptional profiling of Wnt3a mutants identifies Sp transcription factors as essential effectors of the Wnt/β-catenin pathway in neuromesodermal stem cells.
    Authors: Kenneth Et al.
    PLoS One  2014;9:e87018
  398. Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells.
    Authors: Peter Et al.
    Nat Protoc  2014;9:1514-31
  399. Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors.
    Authors: Naujok Et al.
    BMC Res Notes  2014;7:273
  400. Mouse liver repopulation with hepatocytes generated from human fibroblasts.
    Authors: Zhu Et al.
    Nature  2014;508:93
  401. Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells.
    Authors: Shelton Et al.
    Dis Model Mech  2014;3:516
  402. A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence.
    Authors: Muthusamy Et al.
    Stem Cell Reports  2014;3:169
  403. Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts.
    Authors: Orlova Et al.
    Arterioscler Thromb Vasc Biol  2014;34:177
  404. A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation.
    Authors: Wu Et al.
    Stem Cell Res  2014;289:20193
  405. SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development.
    Authors: Skelton Et al.
    J Cachexia Sarcopenia Muscle  2014;13:172
  406. Determination of Functional Activity of Human iPSC-Derived Hepatocytes by Measurement of CYP Metabolism.
    Authors: Yoon-Young Et al.
    Methods Mol Biol  2014;1357:383-94
  407. In Vitro Modeling of Alcohol-Induced Liver Injury Using Human-Induced Pluripotent Stem Cells.
    Authors: Yoon-Young Et al.
    Methods Mol Biol  2014;1353:271-83
  408. Hematopoietic differentiation of pluripotent stem cells in culture.
    Authors: Mitchell J Et al.
    Methods Mol Biol  2014;1185:181-94
  409. Dual-SMAD Inhibition/WNT Activation-Based Methods to Induce Neural Crest and Derivatives from Human Pluripotent Stem Cells.
    Authors: Lorenz Et al.
    Methods Mol Biol  2013;1307:329-43

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iPSC differentiation
By Ricardo Malvicini on 09/26/2021
Species: Human

Used for the differentiation of Human IPSc to cardiomyocites (concentrarion of 5uM)

cells treatment
By Anonymous on 12/04/2017

Treating cells

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