WSTF Antibody
Novus Biologicals | Catalog # NB600-279
![Western Blot: WSTF Antibody [NB600-279]](https://resources.rndsystems.com/images/products/WSTF-Antibody-Western-Blot-NB600-279-img0006.jpg "Western Blot: WSTF Antibody [NB600-279]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Williams Syndrome Transcription Factor using the numbering given in SwissProt entry Q9UIG0 (GeneID 9031).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for WSTF Antibody
Western Blot: WSTF Antibody [NB600-279]
Western Blot: WSTF Antibody [NB600-279] - Whole cell lysate (50 ug) from HCT116, HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WSTF antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes![Immunohistochemistry-Paraffin: WSTF Antibody [NB600-279]](https://resources.rndsystems.com/images/products/WSTF-Antibody-Immunohistochemistry-Paraffin-NB600-279-img0007.jpg "Immunohistochemistry-Paraffin: WSTF Antibody [NB600-279]")
Immunohistochemistry-Paraffin: WSTF Antibody [NB600-279]
Immunohistochemistry-Paraffin: WSTF Antibody [NB600-279] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-WSTF used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunoprecipitation: WSTF Antibody [NB600-279]](https://resources.rndsystems.com/images/products/WSTF-Antibody-Immunoprecipitation-NB600-279-img0008.jpg "Immunoprecipitation: WSTF Antibody [NB600-279]")
Immunoprecipitation: WSTF Antibody [NB600-279]
Immunoprecipitation: WSTF Antibody [NB600-279] - Detection of human WSTF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WSTF antibody NB600-279(lot NB600-279-2) used for IP at 3 ug per reaction. WSTF was also immunoprecipitated by a previous lot of this antibody (lot NB600-279-1). For blotting immunoprecipitated WSTF, NB600-279 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for WSTF Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 -1:500
Immunohistochemistry-Paraffin
1:100 -1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: WSTF
Alternate Names
bromodomain adjacent to zinc finger domain, 1B, williams-Beuren syndrome chromosomal region 10 protein
Entrez Gene IDs
9031 (Human)
Gene Symbol
BAZ1B
UniProt
Additional WSTF Products
Product Documents for WSTF Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for WSTF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for WSTF Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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