XPB Antibody - BSA Free

Novus Biologicals | Catalog # NB100-61060

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rabbit, Virus

Cited:

Human, Frog - Xenopus (African Clawed Frog), Virus

Predicted:

Bovine (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all XPB Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).

Reactivity Notes

Use in Epstein-Barr virus reported in scientific publication (PMID: 32434920). Rabbit reactivity reported from CiteAb.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XPB Antibody - BSA Free

Western Blot: XPB Antibody [NB100-61060]

Western Blot: XPB Antibody [NB100-61060]

Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: XPB Antibody [NB100-61060]

Immunoprecipitation: XPB Antibody [NB100-61060]

Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody NB100-61060 used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody NB100-61059. For blotting immunoprecipitated ERCC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
XPB Antibody

Western Blot: XPB Antibody [NB100-61060] -

nb100-61060_rabbit-polyclonal-xpb-antibody-2092023181154.jpg
XPB Antibody

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - Effects of triptolide & histone depletion on condensation using mouse sperm.(A) Representative fluorescence images of chromatid assembly at steady state with mouse sperm nuclei in egg extracts (180 min after sperm nuclei addition) in the presence of indicated inhibitors. Triptolide or DMSO control was added at 25 min after nuclei addition. Triptolide was added at 50 μM. (B) Western blot for histone H3, CAP-E, & XPB in histone H4K12ac or IgG-depleted extracts in the presence of triptolide (TPL) or DMSO. Relative percentage of protein remaining after indicated treatments is shown.Figure 5—figure supplement 1—source data 1.Source data for Figure 5—figure supplement 1B.Source data for Figure 5—figure supplement 1B. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
XPB Antibody

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
XPB Antibody

Western Blot: XPB Antibody [NB100-61060] -

Western Blot: XPB Antibody [NB100-61060] - The TFIIH complex is required for the enrichment of condensins on chromosomes.(A) Left: Representative immunofluorescence images of Xenopus sperm nuclei incubated with Mock or CAP-E depleted extracts for 180 min. Chromatids were labeled with Hoechst & anti-XPB antibodies. Right: Quantification of fluorescence intensity of XPB on chromatin, normalized to Mock depleted condition. n = 24 structures for each condition. Error bars represent SD, & ‘NS’ indicates no statistically significant difference (p > 0.001). A.U., arbitrary units. Two biological replicates were performed, quantified structures are from a single experiment. (B) Western blot for indicated proteins that copurify with anti-XPB or IgG beads isolated from HSS extracts. IP, immunoprecipitate. (C) Western blot for indicated proteins that copurify with anti-CAP-E or Mock-treated beads isolated from HSS extracts. For condensin subunit detection, the blot was probed simultaneously with the indicated antibodies. Beads were coupled to either anti-CAP-E or pre-immune serum (“Mock”) & processed as indicated in the methods. IP, immunoprecipitate. (D) Representative immunofluorescence images of Xenopus sperm nuclei incubated with HSS extracts for 180 min. Chromatids were labeled with Hoechst & co-stained with anti-XPB & anti-CAP-E antibodies. Images of three representative individual chromatids are shown.Figure 3—figure supplement 1—source data 1.Source data for Figure 3—figure supplement 1B & C.Source data for Figure 3—figure supplement 1B & C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for XPB Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:32434920).

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-61060 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XPB

ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.

Long Name

Xeroderma Pigmentosum B

Alternate Names

ERCC3, GTF2H, RAD25, TFIIH

Entrez Gene IDs

2071 (Human); 13872 (Mouse)

Gene Symbol

ERCC3

UniProt

NP_000113.1 (Human)

Additional XPB Products

Product Documents for XPB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for XPB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for XPB Antibody - BSA Free

Customer Reviews for XPB Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Showing  1 - 2 of 2 reviews Showing All
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  • XPB Antibody - BSA Free
    Name: Shinichi Oka
    Application: Western Blot
    Sample Tested: primary neonatal ventricular cardiomyocytes
    Species: Rat
    Verified Customer | Posted 04/23/2026
    Protein lysates were analyzed by immunoblotting using an anti-XPB antibody (NB100-61060). Multiple bands were detected, with relatively strong signals both above and below the 75 kDa marker.
    XPB Antibody - BSA Free NB100-61060
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • XPB Antibody
    Name: Christophe Martella
    Application: Chromatin Immunoprecipitation
    Sample Tested: CD8 T lymphocytes and human lymphocytes, whole cell lysate
    Species: Human
    Verified Customer | Posted 04/23/2019
    chIP on T lymphocyte

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