YM 58483

Tocris Bioscience | Catalog # 3939

Inhibitor of SOCE in non-excitable cells
Tocris Bioscience

Key Product Details

Description

Inhibitor of SOCE in non-excitable cells

Alternative Names

Btp2
View all Other Calcium Channel Inhibitors »

Product Description

YM 58483 is a blocker of store-operated Ca2+ entry (SOCE), which mediates the activation of non-excitable cells (e.g. lymphocytes). Inhibits calcium release-activated calcium (CRAC) channels; suppresses thapsigargin-induced sustained Ca2+ influx (IC50 = 100 nM). Displays immuno-modulatory and anti-inflammatory effects; suppresses cytokine production and proliferation of T cells in vitro.

Product Specifications for YM 58483

Molecular Weight

421.32

Formula

C15H9F6N5OS

Storage

Store at +4°C

Purity

≥99% (HPLC)

Chemical Name

N-[4-[3,5-Bis(trifluoromethyl)-1H-pyrazol-1-yl]phenyl]-4-methyl-1,2,3-thiadiazole-5-carboxamide

CAS Number

223499-30-7

PubChem ID

2455

InChI Key

XPRZIORDEVHURQ-UHFFFAOYSA-N

SMILES

O=C(C3=C(C)N=NS3)NC1=CC=C(N2N=C(C(F)(F)F)C=C2C(F)(F)F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.13 100
Ethanol 42.13 100

Preparing Stock Solutions for YM 58483

The following data is based on the product molecular weight 421.32.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.37 mL 11.87 mL 23.73 mL
5 mM 0.47 mL 2.37 mL 4.75 mL
10 mM 0.24 mL 1.19 mL 2.37 mL
50 mM 0.05 mL 0.24 mL 0.47 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 48 publications citing the usage of this product.

Product Documents for YM 58483

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for YM 58483

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for YM 58483 (3)

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  • Inhibits calcium entry in mast cells.
    Name: ANANTH KUMAR KAMMALA
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: LAD2 - mast cells
    Verified Customer | Posted 01/30/2021
    YM 58483 was used to study the role of the MRGPRX2 receptor in store-operated calcium entry using human mast cells (LAD2 cells). At a concentration of 0.3 and 1 uM of YM58483 inhibited the calcium entry induced by cortistatin in mast cells.
    YM 58483 3939
  • BTP2 attenuates store-operated Ca2+ entry
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 07/10/2019
    HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min, washed and kept aside for 20 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and fluorescence at 510 nm was expressed as 340/380 ratio. Intracellular Ca2+ stores were depleted with 30 μM BHQ which promotes store-operated Ca2+ entry into the cells upon addition of 1 mM extracellular Ca2+. Preincubation of cells with 10 μM BTP2 (YM 58483) for 10 min attenuates this Ca2+ entry.
    YM 58483 3939
  • Store-operated calcium entry blocker
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Cell Line/Tissue: Brain slice
    Verified Customer | Posted 01/31/2018
    Used to block SOCE mechanism but no effect was seen. Unsure about efficacy of the compound, it might be a lack of SOCE involvement in the biological process studied here.

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