ZBTB22 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-92603
![Western Blot: ZBTB22 Antibody [NBP1-92603]](https://resources.rndsystems.com/images/products/ZBTB22-Antibody-Western-Blot-NBP1-92603-img0004.jpg "Western Blot: ZBTB22 Antibody [NBP1-92603]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SAVGSGERRGGGPVFPAPVVGSGGATSGKLLLEADELCDDGGDGRGAVVPGAGLRRPTYTPPSIMPQKHWVYVKRGGNCPAP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZBTB22 Antibody - BSA Free
Western Blot: ZBTB22 Antibody [NBP1-92603]
Western Blot: ZBTB22 Antibody [NBP1-92603] - Analysis in control (vector only transfected HEK293T lysate) and ZBTB22 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![ZBTB22 Antibody - BSA Free Western Blot: ZBTB22 Antibody - BSA Free [NBP1-92603]](https://resources.rndsystems.com/images/products/nbp1-92603_rabbit-polyclonal-zbtb22-antibody-30520259562023.jpg "Western Blot: ZBTB22 Antibody - BSA Free [NBP1-92603]")
Western Blot: ZBTB22 Antibody - BSA Free [NBP1-92603]
Analysis in control (vector only transfected HEK293T lysate) and ZBTB22 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).![ZBTB22 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: ZBTB22 Antibody - BSA Free [NBP1-92603]](https://resources.rndsystems.com/images/products/nbp1-92603_rabbit-polyclonal-zbtb22-antibody-295202516162211.jpg "Immunocytochemistry/ Immunofluorescence: ZBTB22 Antibody - BSA Free [NBP1-92603]")
Immunocytochemistry/ Immunofluorescence: ZBTB22 Antibody - BSA Free [NBP1-92603]
Staining of human cell line U-2 OS shows localization to nucleoplasm.![ZBTB22 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZBTB22 Antibody - BSA Free [NBP1-92603]](https://resources.rndsystems.com/images/products/nbp1-92603_rabbit-polyclonal-zbtb22-antibody-25620259121956.jpg "Chromatin Immunoprecipitation-exo-Seq: ZBTB22 Antibody - BSA Free [NBP1-92603]")
Chromatin Immunoprecipitation-exo-Seq: ZBTB22 Antibody - BSA Free [NBP1-92603]
ChIP-Exo-Seq composite graph for Anti-ZBTB22 (NBP1-92603) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for ZBTB22 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZBTB22
Alternate Names
BING1ZNF297, fru, fruitless, Protein BING1, ZBTB22AZNF297A, zinc finger and BTB domain containing 22, zinc finger and BTB domain-containing protein 22, Zinc finger protein 297Zinc finger and BTB domain-containing protein 22A
Gene Symbol
ZBTB22
Additional ZBTB22 Products
Product Documents for ZBTB22 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZBTB22 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ZBTB22 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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