ZFP41 Antibody - BSA Free

Western Blot: ZFP41 Antibody [NBP2-83801]

Western Blot: ZFP41 Antibody [NBP2-83801] - Host: Rabbit. Target Name: ZFP41. Sample Type: Liver Tumor lysates. Antibody Dilution: 1.0ug/ml

Western Blot: ZFP41 Antibody - BSA Free [NBP2-83801] -

YTHDF3‐mediated m6A modification of ZFP41 mRNA and decays its mRNA stability. (A) qPCR results showed that efficiencies of siRNA‐mediated knockdown of common m6A regulators in MHCC‐97H cells. (B) qPCR results showed that expression of ZFP41 after silencing these m6A regulators in MHCC‐97H cells. (C) Overexpression of YTHDF3 notably suppress ZFP41 expression on transcription level in MHCC‐97H and Hep3B cells. (D) Knockdown of YTHDF3 obviously increase ZFP41 expression on mRNA level in MHCC‐97H and Hep3B cells. (E) Western blots results demonstrated that the ZFP41 protein level in MHCC‐97H and HLF cells after silencing YTHDF3 with siRNA of YTHDF3. (F) Western blots results demonstrated that the ZFP41 protein level in Hep3B cells after overexpression of YTHDF3. (G) The diagram of the potential site of m6A modification on the CDS (Coding Sequence) area. (H) The luciferase activity in both MHCC‐97H and Hep3B cells cotransfected with relative plasmids. (I) MeRIP results showed that ZFP41‐wt or ZFP41‐mut in both MHCC‐97H and Hep3B cells. (J) The rate of ZFP41 mRNA degradation in MHCC‐97H and Hep3B cells with YTHDF3 overexpression or knockdown. (K) The binding of ZFP41 mRNA and YTHDF3 was tested in MHCC‐97H and Hep3B cells by RIP‐qPCR analyses. (L) The relationship between ZFP41 and YTHDF3 was confirmed by RNA pull‐down assay. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39473907), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ZFP41 Antibody - BSA Free [NBP2-83801] -

ZFP41 suppresses the proliferation and invasion of HCC cells by inhibiting Snail expression and EMT pathway. (A) Representative images of subcutaneous xenograft tumors formation of the Vector group, oeZFP41 group and oeZFP41+Snail group in MHCC‐97H cells. The dissected tumors from two groups were photographed. (B) Volumes and Weights of subcutaneous xenograft tumors in the Vector group, oeZFP41 group and oeZFP41+Snail group. (C and D) The lung fluorescence and the number of lung metastatic nodules was calculated in each group. (E) HE staining of lung tissue in each group were presented. (F) Western blots results showed the protein level of Snail and EMT‐related targets with Vector, oeZFP41, and oeZFP41+Snail groups in MHCC‐97H and Hep3B cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39473907), licensed under a CC-BY license. Not internally tested by Novus Biologicals.