Zika virus (H/PF/2013) NS1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13204
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of Zika virus (H/PF/2013) NS1 (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
Reactivity Notes
Zika virus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Zika virus (H/PF/2013) NS1 Antibody - BSA Free
Western Blot: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204]
Western Blot: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus (H/PF/2013) NS1 antibody (NBP3-13204) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204]
Immunocytochemistry/Immunofluorescence: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204] - Immunofluorescent analysis of non-infected and infected vero cells using Zika virus (H/PF/2013) NS1 antibody (NBP3-13204). Green: Zika virus (H/PF/2013) NS1 antibody (NBP3-13204) diluted at 1:500.Western Blot: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204]
Western Blot: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204] - Non-infected (-) and infected (+) Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus (H/PF/2013) NS1 antibody (NBP3-13204) diluted at 1:2500.
Sandwich ELISA: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13204 as capture antibody at concentration of 5 ug/mL and NBP3-13589 as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA: Zika virus (H/PF/2013) NS1 Antibody [NBP3-13204] - Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using NBP3-13589 as capture antibody at concentration of 5 ug/mL and NBP3-13204 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Applications for Zika virus (H/PF/2013) NS1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Zika Virus (H/PF/2013) NS1
Long Name
Zika Virus Polyprotein
Additional Zika Virus (H/PF/2013) NS1 Products
Product Documents for Zika virus (H/PF/2013) NS1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Zika virus (H/PF/2013) NS1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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