ZNF232 Antibody (PCRP-ZNF232-2B3)
Novus Biologicals | Catalog # NBP3-23598
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # PCRP-ZNF232-2B3
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Product Specifications
Immunogen
Recombinant fragment (around aa29-109) of human ZNF232 protein
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for ZNF232 Antibody (PCRP-ZNF232-2B3)
![ZNF232 Antibody (PCRP-ZNF232-2B3) Flow Cytometry: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -](https://resources.rndsystems.com/images/products/nbp3-23598_mouse-znf232-mab-pcrp-znf232-2b3-2412025863616.jpg "Flow Cytometry: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -")
Flow Cytometry: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -
Flow Cytometry: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] - Flow cytometric analysis of PFA-fixed HeLa cells. ZNF232 Antibody (PCRP-ZNF232-2B3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).![ZNF232 Antibody (PCRP-ZNF232-2B3) Protein Array: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -](https://resources.rndsystems.com/images/products/nbp3-23598_mouse-znf232-mab-pcrp-znf232-2b3-241202587237.jpg "Protein Array: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -")
Protein Array: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -
Protein Array: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZNF232 Antibody (PCRP-ZNF232-2B3).![ZNF232 Antibody (PCRP-ZNF232-2B3) Immunohistochemistry-Paraffin: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -](https://resources.rndsystems.com/images/products/nbp3-23598_mouse-znf232-mab-pcrp-znf232-2b3-2412025872363.jpg "Immunohistochemistry-Paraffin: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -")
Immunohistochemistry-Paraffin: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] -
Immunohistochemistry-Paraffin: ZNF232 Antibody (PCRP-ZNF232-2B3) [NBP3-23598] - Formalin-fixed, paraffin-embedded human tumor of unknown origin stained with ZNF232 Antibody (PCRP-ZNF232-2B3). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.Applications for ZNF232 Antibody (PCRP-ZNF232-2B3)
Application
Recommended Usage
Flow Cytometry
1-2ug/million cells
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ZNF232
Alternate Names
zinc finger protein 500, Zinc finger protein with KRAB and SCAN domains 18, ZKSCAN18KIAA0557
Gene Symbol
ZNF232
Additional ZNF232 Products
Product Documents for ZNF232 Antibody (PCRP-ZNF232-2B3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ZNF232 Antibody (PCRP-ZNF232-2B3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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