ZNF96 Overexpression Lysate

Name: ZNF96 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-18267
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-18267

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for ZNF96 Overexpression Lysate

ZNF96 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC207900

Accession#: NM_001039643

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Type

Overexpression

Scientific Data Images for ZNF96 Overexpression Lysate

Western Blot: ZNF96 Overexpression Lysate [NBL1-18267]

Western Blot: ZNF96 Overexpression Lysate (Adult Normal) [NBL1-18267] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZNF96.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: ZNF96

ZNF96 may be involved in transcriptional regulation

Alternate Names

KIAA0426dJ29K1.2, ZFP96, zinc finger and SCAN domain containing 12, Zinc finger protein 305ZNF305, Zinc finger protein 96ZNF96zinc finger and SCAN domain-containing protein 12, ZNF29K1

Gene Symbol

ZSCAN12

Additional ZNF96 Products

Product Documents for ZNF96 Overexpression Lysate

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Certificate of Analysis

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Product Specific Notices for ZNF96 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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