ZP4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86823

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ZP4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FAVNLNQEATSPPVLIAWDNQGLLHELQNDSDCGTWIRKGPGSSVVLEATYSSCYVTEWDSHYIMPVGVEGAGAAEHKVVTERKLLKCPMDLLARDAPDTDWCDSIPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZP4 Antibody - BSA Free

Immunohistochemistry-Paraffin: ZP4 Antibody [NBP1-86823]

Immunohistochemistry-Paraffin: ZP4 Antibody [NBP1-86823]

Immunohistochemistry-Paraffin: ZP4 Antibody [NBP1-86823] - Staining of human ovary shows cytoplasmic positivity in oocytes.
ZP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
ZP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Staining of human lymph node shows no positivity in non-germinal center cells as expected.
ZP4 Antibody - BSA Free Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Immunohistochemistry-Paraffin: ZP4 Antibody - BSA Free [NBP1-86823]

Staining of human liver shows no positivity in hepatocytes as expected.

Applications for ZP4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZP4

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. (provided by RefSeq)

Alternate Names

zinc finger and homeodomain protein 1, zinc fingers and homeobox 1, zinc fingers and homeoboxes 1, zinc fingers and homeoboxes protein 1, zinc-fingers and homeoboxes 1

Entrez Gene IDs

57829 (Human)

Gene Symbol

ZP4

UniProt

Q12836 (Human)

Additional ZP4 Products

Product Documents for ZP4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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