Recombinant Active Protein Kinases

Protein kinases play critical roles in signal transduction, and their physiological activities in cellular processes are important in defining signaling pathways. Likewise, unregulated activities of protein kinases are of particular interest in understanding pathological pro­cesses such as cancer and chronic inflam­matory diseases. Protein kinases often have multiple physiological substrates and cellular functions. Therefore, specific inhibitors of pro­tein kinases are of interest in drug de­velopment. R&D Systems now offers a wide range of new recombinant active kinases that can be used in studies of their activities and inhibitors. These include members of the Src family, and kinases involved in the PI 3-kinase and MAP kinase signaling pathways.

Figure 1: DuoSet IC Kinase Assay Western Blot and Data
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Figure 1. The phosphorylation of inactive p38 alpha by recombinant human active MKK6 was quantified at the indicated timepoints using the Phospho-p38 alpha (T180/Y182) DuoSet IC Kinase Assay (Catalog # DYC869). The same samples were also immunoblotted (insert) with either anti-phospho-p38 MAPK polyclonal antibody (Catalog # AF869) or anti-p38 alpha monoclonal antibody (Catalog # MAB869).
Category Active Kinase Species Catalog # Assay Substrate for Active Kinase
Cell Cycle Chk1 Human 1630-KS KKKVSRSGLYRSPSMPENLNRPR
Chk2 Human 1358-KS KKKVSRSGLYRSPSMPENLNRPR
NEK2 Human 3706-KS Myelin basic protein (MBP)
MAP Kinase ASK1 Human 3575-KS Myelin basic protein (MBP)
ERK1 Human 1879-KS Myelin basic protein (MBP)
JNK1 Mouse 1776-KS Recombinant Activating Transcription Factor (ATF)-2
MAPKAPK2 Human 3705-KS RRLNRQLSVA-amide
p38 alpha Human 1806-P3 Myelin basic protein (MBP)
Raf-1 Human 3708-KS Myelin basic protein (MBP)
PI 3-kinase Akt1 Human 1775-KS RPRAATF
GSK-3 beta Human 2506-KS YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE
p70 S6 Kinase Human 896-KS CKRRRLASLR
SGK1 Human 3200-KS RPRAATF
Src Lck Human 3704-KS Poly (Glu:Tyr, 4:1)
Src Viral 3389-KS KVEKIGEGTYGVVYK
T cell Receptor ZAP70 Human 3709-KS Poly (Glu:Tyr, 4:1)