Rat/Mouse Cytochrome c Quantikine ELISA Kit

R&D Systems | Catalog # MCTC0

R&D Systems
100% Guarantee

Key Product Details

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Cell Lysates (50 µL), Subcellular Fractions (50 µL)

Sensitivity

0.5 ng/mL

Assay Range

0.78-25 ng/mL (Cell Lysates, Subcellular Fractions)
View all Cytochrome c ELISA Kits »

Product Summary for Rat/Mouse Cytochrome c Quantikine ELISA Kit

The Quantikine Rat/Mouse Cytochrome c Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat and mouse cytochrome c. It contains natural rat cytochrome c and antibodies raised against cytochrome c, and because of the 100% sequence identity of rat and mouse cytochrome c, this kit can be used to accurately quantitate natural rat or mouse cytochrome c in cell lysates and subcellular fractions.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse, Rat

Specificity

This kit recognizes both the reduced and oxidized forms of natural rat and mouse cytochrome c.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Lysates, Subcellular Fractions

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.61 8.82 13.4 1.72 8.91 13.5
Standard Deviation 0.02 0.09 0.19 0.06 0.31 0.59
CV% 1.24 1.02 1.42 3.49 3.48 4.37

Recovery for Rat/Mouse Cytochrome c Quantikine ELISA Kit

The recovery of rat/mouse Cytochrome c spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
PBS/0.01% Saponin (n=2) 94 90-98
PBS/0.05% Tween 20 (n=2) 101 100-102
PBS/0.5% Triton X-100 (n=2) 100 96-106
PBS/1% Triton X-100 (n=2) 98 95-101

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat/mouse Cytochrome c in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse/Rat Cytochrome c ELISA Linearity

Scientific Data Images for Rat/Mouse Cytochrome c Quantikine ELISA Kit

Mouse/Rat Cytochrome c ELISA Standard Curve

Mouse/Rat Cytochrome c ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytochrome c

Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Rat/Mouse Cytochrome c Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat/Mouse Cytochrome c Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Rat/Mouse Cytochrome c Quantikine ELISA Kit

Customer Reviews for Rat/Mouse Cytochrome c Quantikine ELISA Kit (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Rat/Mouse Cytochrome c Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Rat/Mouse Cytochrome c Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: cytochrome c standard
    Verified Customer | Posted 05/19/2017
    Rat/Mouse Cytochrome c Quantikine ELISA Kit MCTC0

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for Rat/Mouse Cytochrome c Quantikine ELISA Kit (MCTC0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 75 µL Conjugate
  3.   Add 75 µL of Conjugate to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Substrate Solution
  6.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    7. 100 µL Stop Solution
  7.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits