Blogs for February 2018

Tips for Adult Stem Cell Differentiation using MimEX™ Reagents

Thursday, February 15, 2018 - 10:15

MimEX™ Tissue Model Systems provide a robust system to generate accessible ex vivo 3-D tissue from epithelial-derived organs. This short video focuses on tips to optimize the differentiation of MimEX Tissue in an air-liquid interface. This includes helpful information on harvesting adult stem cells for differentiation, optimizing the setup of transwell inserts, and monitoring and identifying successful versus unsuccessful differentiation in the air-liquid interface system.

In this protocol you will learn:

  • Optimal harvesting density of adult stem cells
  • Ideal structure of a healthy adult stem cell colony
  • Troubleshooting fibroblast contamination when isolating adult stem cells from biopsy tissue
  • Tips for working with transwell inserts
  • Expected cytoarchitectural changes...

Bio-Techne Named Researchers' Choice by CiteAb

Wednesday, February 7, 2018 - 12:49
Press Release Bio-Techne

Bio-Techne is honored to receive the CiteAb Researchers’ Choice Award for 2018! The annual awards aim to celebrate the very best suppliers and individuals in the research reagent sector worldwide.

Bio-Techne receives CiteAb Researchers Choice Award 2018

The award of Researchers’ Choice reflects what researchers’ really think about the companies they get a wide range of reagents from for their work. It was noted that Bio-Techne...

Bio-Techne’s Ella Added to BioAgilytix Portfolio of Industry-Leading Immunoassay Platforms

Wednesday, February 7, 2018 - 10:49
lab scientists press release

Bio-Techne’s ProteinSimple-branded Ella™ platform has recently been added to the suite of advanced immunoassay technology at BioAgilytix, a leading provider of contract bioanalytical testing services with specialization in large molecule bioanalysis.

Ella joins her other immunoassay platform from ProteinSimple, PeggySue®, along with several other bioanalytical technologies and automation systems in the BioAgilytix core immunoassay services in their laboratories....

MimEX™ Cell Plating Procedure

Saturday, February 3, 2018 - 09:57

This protocol video describes the MimEX Cell Plating Procedure. This procedure is important when using the MimEX Tissue Model System from R&D Systems. First, the MimEX Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue-specific adult epithelial stem cells. It is also used to optimize the seeding distribution of adult stem cells on top of the fibroblasts monolayer. This distribution helps to maintain the health and rapid proliferation rate of adult epithelial stem cells using MimEX Tissue Model Systems.

Briefly, the cell plating procedure consists of three shakes of the plate backward and forward and three shakes of the plate side-to-side. This procedure is repeated three times before...

Single-cell Profiling with Mass Cytometry: An Overview of Technology and Current Research Applications On-Demand

Thursday, February 1, 2018 - 12:21

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes. Readout is by time-of-flight mass spectrometry. This allows for the combination of many more antibodies (over 30) for phenotypic profiling and functional analysis of single cells, without significant overlap between channels. Mass cytometry has now been applied to various research areas including basic immunology, neuroscience, immuno-oncology, drug discovery, and stem cell research. While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level. These steps include experimental planning, sample and reagent preparation,...

Stem Cells in the News - February 2018

Thursday, February 1, 2018 - 11:42
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

ScienceDaily

For the first time, trophoblast stem cells have been isolated and cultured by researchers in Japan. These cells line the outer layer of the blastocyst and eventually become...