Assessing cell health is an important workflow step in many cell culture systems and can be monitored using assays for the quantification of cell proliferation, cell viability, apoptosis, and DNA damage. Cell proliferation and viability can be quantified using fluorescent and non-fluorescent cell-permeable dyes, as well as using assays that measure metabolic activity. Apoptosis is studied using kits that assess apoptosis-associated cellular events, including TUNEL Assays, Annexin V staining, and mitochondrial membrane disruption. In addition, several assays are available to assess the mechanisms underlying DNA damage and repair.
Determining cell viability is an important workflow step in many cell culture systems. R&D Systems offers a variety of reagents for studying cell proliferation, viability, and cytotoxicity including fluorescent and non-fluorescent reporter dyes and MTT assays.
DNA damage can result from cellular metabolism, routine errors in DNA replication or recombination, and exposure to exogenous genotoxic agents, such as radiation and oxidative stress. R&D Systems offers assays to assess the mechanisms underlying DNA damage and repair.