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How to Non-invasively Monitor Stem Cell Differentiation using Luminex® Technology
Monday, September 12, 2016 - 13:32
Joy Aho, PhD, Manager of Stem Cell Research and Development, Bio-Techne
Richard Fuerstenberg, Manager of Multiplex Development, Bio-Techne
In this webinar, Bio-Techne scientists, Richard Fuerstenberg and Joy Aho, discuss a novel method for monitoring stem cell pluripotency, expansion, and differentiation in vitro. This technology utilizes non-invasive sampling of cell culture supernatants to identify and follow changes in key secreted growth factors and cytokines. Using Proteome Profiler™ Antibody Arrays and Luminex® Assays, researchers can quickly monitor changes in cell phenotype without sacrificing yield. These techniques can be used to minimize cross-experiment variability during differentiation as well as to identify key analytes that could be used to predict or direct cell expansion or differentiation.