5-HT2B Antibody - BSA Free

Novus Biologicals | Catalog # NLS1111

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey, Primate

Cited:

Human

Predicted:

Bovine (93%), Chimpanzee (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT2B Primary Antibodies »

Product Specifications

Immunogen

Synthetic 15 amino acid peptide from 2nd extracellular domain of human 5-HT2B.

Epitope

2nd extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (80%), Hamster (80%), Equine (80%), Rabbit (80%), Porcine (80%).

Localization

Cell Membrane

Specificity

Human 5-HT2B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for 5-HT2B Antibody - BSA Free

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Analysis of anti-5HT2B Receptor antibody with human brain, cortex.
Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody - BSA Free [NLS1111]

Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for 5-HT2B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

4 - 6 ug/ml
Application Notes
Use in Proximity ligation assay reported in scientific literature (PMID: 31091436). ICC: Does not work..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: 5-HT2B

5-HT2B, a Serotonin Receptor, activates phospholipase C upon binding serotonin, which results in a rise in intracellular calcium. It is known that 5-HT2B regulates cardiovascular function during development and in adulthood; mice lacking functional 5-HT2B receptors died of heart defects during gestation or neonatally, and adult mutant mice displayed cardiopathy, including myocyte disarray and ventricular dilation. Abnormal vascular proliferation causes an increase in pulmonary blood pressure, which is associated with an increase in 5-HT2B receptor, resulting in the development of pulmonary hypertension. It has also been reported that the 5-HT2B is involved in cell-cycle regulation via interaction with tyrosine kinase pathways. 5-HT2B receptors may have a role in the initiation of migraine headaches, and selective antagonists may prove therapeutic in migraine treatment. 5-HT2B receptor is expressed in many tissues including human liver, kidney, lung, heart, pancreas, spleen, brain, spinal cord, gastrointestinal tract, placenta, and coronary and pulmonary arteries. It has also been reported in embryonic mouse heart and spinal cord. ESTs have been isolated from heart/melanocyte/uterus, kidney, prostate, skin, and thyroid libraries.

Long Name

5-Hydroxytryptamine Receptor 2B

Alternate Names

5HT2B, HTR2B

Entrez Gene IDs

3357 (Human)

Gene Symbol

HTR2B

UniProt

P41595

Additional 5-HT2B Products

Product Documents for 5-HT2B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT2B Antibody - BSA Free

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Protocols

View specific protocols for 5-HT2B Antibody - BSA Free (NLS1111):

Immunohistochemistry Protocol for 5HT2B Receptor Antibody (NLS1111):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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